DLAT sirna

DLAT siRNA (Mouse)

Cat. Num. AAA8239927

• Gene Names: Dlat; DLTA; PDC-E2; 6332404G05Rik
• Reactivity: Mouse
• Applications: RNA Interference (RNAi)
• Purity: > 97%
• Synonyms: DLAT; DLAT siRNA (Mouse); Dihydrolipoyllysine-residue acetyltransferase component of pyruvate dehydrogenase complex mitochondrial; Dihydrolipoamide acetyltransferase component of pyruvate dehydrogenase complex; Pyruvate dehydrogenase complex component E2; PDC-E2; PDCE2; DLAT sirna

• Host: Synthetic
• Reactivity: Mouse
• Specificity: DLAT siRNA (Mouse) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
• Purity/Purification: > 97%
• Form/Format: Lyophilized powder
• Sequence Length: 642

• Applicable Applications for DLAT sirna: RNA Interference (RNAi)
• Quality Control: Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
• Directions for Use: We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
• Components: We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of mouse DLAT gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
• Preparation and Storage: Shipped at 4 degree C. Store at -20 degree C for one year.

Related Product Information for DLAT sirna

siRNA to inhibit DLAT expression using RNA interference

NCBI and Uniprot Product Information

• NCBI GI #: 257796245
• NCBI GeneID: 235339
• NCBI Accession #: NP_663589.3
• NCBI GenBank Nucleotide #: NM_145614.4
• UniProt Accession #: Q8BMF4; Q8K2G8; Q8R339; Q91ZB1
• Molecular Weight: 67,942 Da
• NCBI Official Full Name: dihydrolipoyllysine-residue acetyltransferase component of pyruvate dehydrogenase complex, mitochondrial
• NCBI Official Synonym Full Names: dihydrolipoamide S-acetyltransferase (E2 component of pyruvate dehydrogenase complex)
• NCBI Official Symbol: Dlat
• NCBI Official Synonym Symbols: DLTA; PDC-E2; 6332404G05Rik
• NCBI Protein Information: dihydrolipoyllysine-residue acetyltransferase component of pyruvate dehydrogenase complex, mitochondrial
• UniProt Protein Name: Dihydrolipoyllysine-residue acetyltransferase component of pyruvate dehydrogenase complex, mitochondrial
• Protein Family: Dihydrolipoyllysine-residue acetyltransferase component of pyruvate dehydrogenase complex
• UniProt Gene Name: Dlat
• UniProt Synonym Gene Names: PDC-E2; PDCE2
• UniProt Entry Name: ODP2_MOUSE

Uniprot Description

DLAT: the E2 component of the pyruvate dehydrogenase complex (PDHC) that catalyzes the overall conversion of pyruvate to acetyl-CoA and CO2. Contains two lipoyl-binding domains. The PDHC plays a major role in controlling the balance between lipid and glucose oxidation depending on substrate availability. The activity of the PDHC is tightly regulated by phosphorylation of the E1 components by the PDHKs. The pyruvate dehydrogenase (PDH) holoenzyme is a multi-enzyme complex (PDHC) that contains 20-30 copies of pyruvate decarboxylase tetramers (2 alpha:2 beta)(E1), 60 copies of dihydrolipoamide acetyltransferase (E2), six homo-dimers of dihydrolipoamide dehydrogenase (E3), plus E3 binding proteins. PDHKs are recruited to the PDHC by binding to a lipoyl group covalently attached to K174 of the inner lipoyl domain of the E2 component.

Protein type: Carbohydrate Metabolism - citrate (TCA) cycle; Mitochondrial; Carbohydrate Metabolism - glycolysis and gluconeogenesis; EC 2.3.1.12; Transferase; Carbohydrate Metabolism - pyruvate

Cellular Component: mitochondrion; pyruvate dehydrogenase complex; mitochondrial pyruvate dehydrogenase complex; myelin sheath

Molecular Function: transferase activity; dihydrolipoyllysine-residue acetyltransferase activity; transferase activity, transferring acyl groups

Biological Process: acetyl-CoA biosynthetic process from pyruvate; metabolic process; tricarboxylic acid cycle; carbohydrate metabolic process; glucose metabolic process; pyruvate metabolic process