DKK1 sirna

DKK1 siRNA (Mouse)

Cat. Num. AAA8231591

• Gene Names: Dkk1; mdkk-1
• Reactivity: Mouse
• Applications: RNA Interference (RNAi)
• Purity: > 97%
• Synonyms: DKK1; DKK1 siRNA (Mouse); Dickkopf-related protein 1; Dickkopf-1; Dkk-1; mDkk-1; DKK1 sirna

• Host: Synthetic
• Reactivity: Mouse
• Specificity: DKK1 siRNA (Mouse) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
• Purity/Purification: > 97%
• Form/Format: Lyophilized powder
• Sequence Length: 272

• Applicable Applications for DKK1 sirna: RNA Interference (RNAi)
• Quality Control: Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
• Directions for Use: We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
• Components: We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of mouse DKK1 gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
• Preparation and Storage: Shipped at 4 degree C. Store at -20 degree C for one year.

Related Product Information for DKK1 sirna

siRNA to inhibit DKK1 expression using RNA interference

NCBI and Uniprot Product Information

• NCBI GI #: 31542557
• NCBI GeneID: 13380
• NCBI Accession #: NP_034181.2
• NCBI GenBank Nucleotide #: NM_010051.3
• UniProt Accession #: O54908; Q80UL5
• Molecular Weight: 29,298 Da
• NCBI Official Full Name: dickkopf-related protein 1
• NCBI Official Synonym Full Names: dickkopf homolog 1 (Xenopus laevis)
• NCBI Official Symbol: Dkk1
• NCBI Official Synonym Symbols: mdkk-1
• NCBI Protein Information: dickkopf-related protein 1
• UniProt Protein Name: Dickkopf-related protein 1
• Protein Family: Dickkopf-related protein
• UniProt Gene Name: Dkk1
• UniProt Synonym Gene Names: Dickkopf-1; Dkk-1; mDkk-1
• UniProt Entry Name: DKK1_MOUSE

Uniprot Description

DKK1: Antagonizes canonical Wnt signaling by inhibiting LRP5/6 interaction with Wnt and by forming a ternary complex with the transmembrane protein KREMEN that promotes internalization of LRP5/6. DKKs play an important role in vertebrate development, where they locally inhibit Wnt regulated processes such as antero- posterior axial patterning, limb development, somitogenesis and eye formation. In the adult, Dkks are implicated in bone formation and bone disease, cancer and Alzheimer disease. Interacts with LRP6. Interacts (via the C-termianl Cys- rich domain) with LRP5 (via beta-propeller regions 3 and 4); the interaction, enhanced by MESD and or KREMEN, antagonizes Wnt- mediated signaling. Placenta. Belongs to the dickkopf family.

Protein type: Inhibitor; Secreted, signal peptide; Secreted

Cellular Component: extracellular space; plasma membrane; extracellular region

Molecular Function: receptor antagonist activity; protein binding; low-density lipoprotein receptor binding

Biological Process: negative regulation of skeletal muscle development; cellular morphogenesis during differentiation; negative regulation of Wnt receptor signaling pathway; response to retinoic acid; Wnt receptor signaling pathway; regulation of receptor internalization; multicellular organismal development; negative regulation of peptidyl-serine phosphorylation; regulation of Wnt receptor signaling pathway; negative regulation of transcription from RNA polymerase II promoter; negative regulation of BMP signaling pathway; negative regulation of mesodermal cell fate specification; mesoderm formation; hair follicle development; endoderm formation; forebrain development; regulation of endodermal cell fate specification; negative regulation of protein complex assembly; embryonic limb morphogenesis; endoderm development