DHDDS sirna

DHDDS siRNA (Mouse)

Cat. Num. AAA8218710

• Gene Names: Dhdds; DS; CIT; HDS; W91638; 3222401G21Rik
• Reactivity: Mouse
• Applications: RNA Interference (RNAi)
• Purity: > 97%
• Synonyms: DHDDS; DHDDS siRNA (Mouse); Dehydrodolichyl diphosphate synthase; Dedol-PP synthase; Cis-isoprenyltransferase; CIT; Cis-IPTase; DHDDS sirna

• Host: Synthetic
• Reactivity: Mouse
• Specificity: DHDDS siRNA (Mouse) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
• Purity/Purification: > 97%
• Form/Format: Lyophilized powder
• Sequence Length: 333

• Applicable Applications for DHDDS sirna: RNA Interference (RNAi)
• Quality Control: Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
• Directions for Use: We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
• Components: We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of mouse DHDDS gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
• Preparation and Storage: Shipped at 4 degree C. Store at -20 degree C for one year.

Related Product Information for DHDDS sirna

siRNA to inhibit DHDDS expression using RNA interference

NCBI and Uniprot Product Information

• NCBI GI #: 27754093
• NCBI GeneID: 67422
• NCBI Accession #: NP_080420.2
• NCBI GenBank Nucleotide #: NM_026144.4
• UniProt Accession #: Q99KU1; Q3UF13; Q8BZ16; Q8BZK8; A3KGL4
• Molecular Weight: 38,637 Da
• NCBI Official Full Name: dehydrodolichyl diphosphate syntase complex subunit Dhdds
• NCBI Official Synonym Full Names: dehydrodolichyl diphosphate synthase
• NCBI Official Symbol: Dhdds
• NCBI Official Synonym Symbols: DS; CIT; HDS; W91638; 3222401G21Rik
• NCBI Protein Information: dehydrodolichyl diphosphate syntase complex subunit Dhdds
• UniProt Protein Name: Dehydrodolichyl diphosphate syntase complex subunit Dhdds
• Protein Family: Dehydrodolichyl diphosphate synthase complex
• UniProt Gene Name: Dhdds
• UniProt Synonym Gene Names: CIT; Cis-IPTase
• UniProt Entry Name: DHDDS_MOUSE

Uniprot Description

DHDDS: Catalyzes cis-prenyl chain elongation to produce the polyprenyl backbone of dolichol, a glycosyl carrier-lipid required for the biosynthesis of several classes of glycoprotein. Defects in DHDDS are the cause of retinitis pigmentosa type 59 (RP59). RP59 is a retinal dystrophy belonging to the group of pigmentary retinopathies. Retinitis pigmentosa is characterized by retinal pigment deposits visible on fundus examination and primary loss of rod photoreceptor cells followed by secondary loss of cone photoreceptors. Patients typically have night vision blindness and loss of midperipheral visual field. As their condition progresses, they lose their far peripheral visual field and eventually central vision as well. Belongs to the UPP synthase family. 3 isoforms of the human protein are produced by alternative splicing.

Protein type: EC 2.5.1.87; Transferase; Secondary Metabolites Metabolism - terpenoid backbone biosynthesis

Cellular Component: membrane; endoplasmic reticulum

Molecular Function: transferase activity, transferring alkyl or aryl (other than methyl) groups; transferase activity