DGUOK sirna

DGUOK siRNA (Mouse)

Cat. Num. AAA8230704

• Gene Names: Dguok; dGK; dGK1; dGK2; dGK3
• Reactivity: Mouse
• Applications: RNA Interference (RNAi)
• Purity: > 97%
• Synonyms: DGUOK; DGUOK siRNA (Mouse); DGK; Deoxyguanosine kinase mitochondrial; dGK; DGUOK sirna

• Host: Synthetic
• Reactivity: Mouse
• Specificity: DGUOK siRNA (Mouse) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
• Purity/Purification: > 97%
• Form/Format: Lyophilized powder
• Sequence Length: 277

• Applicable Applications for DGUOK sirna: RNA Interference (RNAi)
• Quality Control: Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
• Directions for Use: We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
• Components: We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of mouse DGUOK gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
• Preparation and Storage: Shipped at 4 degree C. Store at -20 degree C for one year.

Related Product Information for DGUOK sirna

siRNA to inhibit DGUOK expression using RNA interference

NCBI and Uniprot Product Information

• NCBI GI #: 247269607
• NCBI GeneID: 27369
• NCBI Accession #: NP_038792.2
• NCBI GenBank Nucleotide #: NM_013764.2
• UniProt Accession #: Q9QX60; Q8CBU2; Q9R0Y2; Q9WUT9
• Molecular Weight: 28,808 Da
• NCBI Official Full Name: deoxyguanosine kinase, mitochondrial isoform 1
• NCBI Official Synonym Full Names: deoxyguanosine kinase
• NCBI Official Symbol: Dguok
• NCBI Official Synonym Symbols: dGK; dGK1; dGK2; dGK3
• NCBI Protein Information: deoxyguanosine kinase, mitochondrial
• UniProt Protein Name: Deoxyguanosine kinase, mitochondrial
• UniProt Gene Name: Dguok
• UniProt Synonym Gene Names: Dgk; dGK
• UniProt Entry Name: DGUOK_MOUSE

Uniprot Description

DGUOK: Required for the phosphorylation of several deoxyribonucleosides and certain nucleoside analogs widely employed as antiviral and chemotherapeutic agents. Defects in DGUOK are the cause of mitochondrial DNA depletion syndrome type 3 (MTDPS3). A disorder characterized by onset in infancy of progressive liver failure, hypoglycemia, increased lactate in body fluids, and neurologic abnormalities including hypotonia, encephalopathy, peripheral neuropathy. Affected tissues show both decreased activity of the mtDNA-encoded respiratory chain complexes and mtDNA depletion. Belongs to the DCK/DGK family. 5 isoforms of the human protein are produced by alternative splicing.

Protein type: EC 2.7.1.113; Mitochondrial; Kinase, other; Nucleotide Metabolism - purine

Cellular Component: mitochondrion; cytoplasm; cytosol; nucleus

Molecular Function: transferase activity; deoxyguanosine kinase activity; nucleoside kinase activity; nucleotide binding; kinase activity; ATP binding

Biological Process: purine deoxyribonucleoside metabolic process; nucleotide biosynthetic process; nucleobase, nucleoside, nucleotide and nucleic acid metabolic process; dGTP metabolic process; phosphorylation; protein amino acid phosphorylation; deoxyribonucleoside monophosphate biosynthetic process