Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Mouse Deoxyguanosine kinase, mitochondrial (DGUOK) Standard Curve.)
Mouse Deoxyguanosine Kinase, Mitochondrial (DGUOK) ELISA Kit | DGUOK elisa kit
Mouse Deoxyguanosine Kinase, Mitochondrial (DGUOK) ELISA Kit
* Allow all reagents to warm to room temperature for at least 30 minutes before opening.
* Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
* Unused wells must be kept desiccated at 4 degree C in the sealed bag provided.
* Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
* It is recommended that all samples and standards be assayed in duplicate or triplicate.
Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Mouse Deoxyguanosine kinase, mitochondrial (DGUOK) Standard Curve.)
Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Mouse Deoxyguanosine kinase, mitochondrial (DGUOK) Standard Curve.)
Background: In mammalian cells, the phosphorylation of purine deoxyribonucleosides is mediated predominantly by two deoxyribonucleoside kinases, cytosolic deoxycytidine kinase and mitochondrial deoxyguanosine kinase. The protein encoded by DGUOK is responsible for phosphorylation of purine deoxyribonucleosides in the mitochondrial matrix. In addition, this protein phosphorylates several purine deoxyribonucleoside analogs used in the treatment of lymphoproliferative disorders, and this phosphorylation is critical for the effectiveness of the analogs. Alternative splice variants encoding different protein isoforms have been described for DGUOK.
NCBI and Uniprot Product Information
Uniprot Description
DGUOK: Required for the phosphorylation of several deoxyribonucleosides and certain nucleoside analogs widely employed as antiviral and chemotherapeutic agents. Defects in DGUOK are the cause of mitochondrial DNA depletion syndrome type 3 (MTDPS3). A disorder characterized by onset in infancy of progressive liver failure, hypoglycemia, increased lactate in body fluids, and neurologic abnormalities including hypotonia, encephalopathy, peripheral neuropathy. Affected tissues show both decreased activity of the mtDNA-encoded respiratory chain complexes and mtDNA depletion. Belongs to the DCK/DGK family. 5 isoforms of the human protein are produced by alternative splicing.
Protein type: EC 2.7.1.113; Kinase, other; Mitochondrial; Nucleotide Metabolism - purine
Chromosomal Location of Human Ortholog: 6 C3|6 35.94 cM
Cellular Component: cytoplasm; cytosol; mitochondrion; nucleus
Molecular Function: ATP binding; deoxyguanosine kinase activity; kinase activity; nucleoside kinase activity; nucleotide binding; transferase activity
Biological Process: dGTP metabolic process; nucleobase, nucleoside, nucleotide and nucleic acid metabolic process; phosphorylation; protein amino acid phosphorylation; purine deoxyribonucleoside metabolic process
Research Articles on DGUOK
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Product Notes
The Mouse DGUOK dguok (Catalog #AAA9717905) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA9717905 ELISA Kit recognizes Mouse DGUOK. It is sometimes possible for the material contained within the vial of "Deoxyguanosine Kinase, Mitochondrial (DGUOK), ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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