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DDX3X sirna

DDX3X siRNA (Mouse)

Gene Names
Ddx3x; Ddx3; Fin14; D1Pas1-rs2
Reactivity
Mouse
Applications
RNA Interference (RNAi)
Purity
> 97%
Synonyms
DDX3X; DDX3X siRNA (Mouse); D1PAS1-RS2; DDX3; DEAD3; ERH; ATP-dependent RNA helicase DDX3X; D1Pas1-related sequence 2; DEAD box RNA helicase DEAD3; mDEAD3; DEAD box protein 3. X-chromosomal; Embryonic RNA helicase; DDX3X sirna
Ordering
For Research Use Only!
Host
Synthetic
Reactivity
Mouse
Specificity
DDX3X siRNA (Mouse) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
Purity/Purification
> 97%
Form/Format
Lyophilized powder
Sequence Length
662
Applicable Applications for DDX3X sirna
RNA Interference (RNAi)
Quality Control
Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
Directions for Use
We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
Components
We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of mouse DDX3X gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
Preparation and Storage
Shipped at 4 degree C. Store at -20 degree C for one year.
Related Product Information for DDX3X sirna
siRNA to inhibit DDX3X expression using RNA interference

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
73,101 Da
NCBI Official Full Name
ATP-dependent RNA helicase DDX3X
NCBI Official Synonym Full Names
DEAD/H (Asp-Glu-Ala-Asp/His) box polypeptide 3, X-linked
NCBI Official Symbol
Ddx3x
NCBI Official Synonym Symbols
Ddx3; Fin14; D1Pas1-rs2
NCBI Protein Information
ATP-dependent RNA helicase DDX3X
UniProt Protein Name
ATP-dependent RNA helicase DDX3X
UniProt Gene Name
Ddx3x
UniProt Synonym Gene Names
D1Pas1-rs2; Ddx3; Dead3; Erh; mDEAD3
UniProt Entry Name
DDX3X_MOUSE

Uniprot Description

DDX3: a multifunctional DEAD box family RNA helicase with diverse cellular functions. DEAD box proteins are characterized by the conserved motif Asp-Glu-Ala-Asp (DEAD), and are involved in several steps of gene expression, such as transcription, maturation of nuclear and mitochondrial mRNA, mRNA export, translation initiation, and ribosome and spliceosome assembly. DDX3 is required for nuclear export of HIV-1 viral transcripts, possibly in a complex with the viral Rev protein and host cofactor CRM1. DDX3 is required for hepatitis C virus (HCV) RNA replication and its expression is downregulated in hepatitis B virus (HBV) associated hepatocellular carcinoma (HCC). May function as a tumor suppressor protein. Its expression inhibits tumor cell colony formation and increases expression of the cdk inhibitor p21 Waf1/Cip1. Low DDX3 expression has been shown in HCC, and aberrant subcellular localization occurs in many squamous cell carcinomas. Reduced DDX3 expression in cultured cells causes a diminished dependence on serum for cell proliferation and changes in cyclin D1 and p21 Waf1/Cip1 expression. Associates with eIF4F to promote translation of selected mRNAs. Is phosphorylated by the mitotic cyclin dependent kinase, cyclin B/cdc2. This phosphorylation may cause a loss of DDX3 function and a concomitant repression of ribosome biogenesis and translation in mitosis. Involved in TBK1 and IKBKE-dependent IRF3 activation leading to IFN-beta induction. Involved in regulation of apoptosis. May be required for activation of the intrinsic but inhibit activation of the extrinsic apoptotic pathway. Regulated by the cell cycle. Maximally expressed in the cytoplasm during G1/S phase and decreased expression during G2/M phase. Located predominantly in nuclear speckles and, at low levels, throughout the cytoplasm. Associates with the outer side of nuclear pore complexes (NPC) and mitochondrial outer membranes. Shuttles between the nucleus and the cytoplasm in an exportin1-dependent manner. Associates with polyadenylated mRNAs in the cytoplasm and the nucleus. Predominantly located in nucleus during G0 phase and in the cytoplasm during G1/S phase. Belongs to the DEAD box helicase family, DDX3 subfamily. Two isoforms of the human protein are produced by alternative splicing.

Protein type: Helicase; RNA-binding; Translation initiation; EC 3.6.4.13; Cell cycle regulation; Spliceosome

Cellular Component: eukaryotic translation initiation factor 3 complex; mitochondrial outer membrane; membrane; mitochondrion; stress granule; cytoplasm; nucleus

Molecular Function: GTPase activity; CTPase activity; hydrolase activity; RNA binding; ATPase activity; nucleotide binding; helicase activity; transcription factor binding; poly(A) binding; ATP-dependent DNA helicase activity; protein binding; DNA binding; nucleic acid binding; translation initiation factor binding; eukaryotic initiation factor 4E binding; nucleoside-triphosphatase activity; mRNA 5'-UTR binding; ATP binding; ribosomal small subunit binding; ATP-dependent RNA helicase activity

Biological Process: stress granule assembly; positive regulation of viral genome replication; transcription, DNA-dependent; positive regulation of translation; apoptosis; positive regulation of apoptosis; immune system process; response to virus; ribosome biogenesis and assembly; positive regulation of caspase activity; negative regulation of caspase activity; positive regulation of cell growth; DNA duplex unwinding; chromosome segregation; positive regulation of translational initiation; regulation of translation; regulation of transcription, DNA-dependent; induction of apoptosis via death domain receptors; negative regulation of translation; RNA secondary structure unwinding; innate immune response; positive regulation of transcription from RNA polymerase II promoter; negative regulation of cell growth; negative regulation of protein complex assembly; mature ribosome assembly; negative regulation of apoptosis

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Product Notes

The DDX3X ddx3x (Catalog #AAA828934) is a siRNA produced from Synthetic and is intended for research purposes only. The product is available for immediate purchase. The DDX3X siRNA (Mouse) reacts with Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's DDX3X can be used in a range of immunoassay formats including, but not limited to, RNA Interference (RNAi). Researchers should empirically determine the suitability of the DDX3X ddx3x for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "DDX3X, siRNA" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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