DDR2 sirna

DDR2 siRNA (Mouse)

Cat. Num. AAA829386

• Gene Names: Ddr2; Ntrkr3; tyro10; AW495251
• Reactivity: Mouse
• Applications: RNA Interference (RNAi)
• Purity: > 97%
• Synonyms: DDR2; DDR2 siRNA (Mouse); NTRKR3; TKT; TYRO10; Discoidin domain-containing receptor 2; Discoidin domain receptor 2; CD167 antigen-like family member B; Neurotrophic tyrosine kinase. receptor-related 3; Receptor protein-tyrosine kinase TKT; Tyrosine-protein kinase TYRO10; CD antigen CD167b; DDR2 sirna

• Host: Synthetic
• Reactivity: Mouse
• Specificity: DDR2 siRNA (Mouse) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
• Purity/Purification: > 97%
• Form/Format: Lyophilized powder
• Sequence Length: 854

• Applicable Applications for DDR2 sirna: RNA Interference (RNAi)
• Quality Control: Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
• Directions for Use: We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
• Components: We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of mouse DDR2 gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
• Preparation and Storage: Shipped at 4 degree C. Store at -20 degree C for one year.

Related Product Information for DDR2 sirna

siRNA to inhibit DDR2 expression using RNA interference

NCBI and Uniprot Product Information

• NCBI GI #: 158508514
• NCBI GeneID: 18214
• NCBI Accession #: NP_072075.2
• NCBI GenBank Nucleotide #: NM_022563.2
• UniProt Accession #: Q62371; B2RSD7
• Molecular Weight: 96,482 Da
• NCBI Official Full Name: discoidin domain-containing receptor 2
• NCBI Official Synonym Full Names: discoidin domain receptor family, member 2
• NCBI Official Symbol: Ddr2
• NCBI Official Synonym Symbols: Ntrkr3; tyro10; AW495251
• NCBI Protein Information: discoidin domain-containing receptor 2
• UniProt Protein Name: Discoidin domain-containing receptor 2
• Protein Family: Discoidin domain-containing receptor
• UniProt Gene Name: Ddr2
• UniProt Synonym Gene Names: Ntrkr3; Tkt; Tyro10; Discoidin domain receptor 2
• UniProt Entry Name: DDR2_MOUSE

Uniprot Description

DDR2: This tyrosine kinase receptor for fibrillar collagen mediates fibroblast migration and proliferation. Contributes to cutaneous wound healing. Belongs to the protein kinase superfamily. Tyr protein kinase family. Insulin receptor subfamily.

Protein type: Protein kinase, TK; Kinase, protein; Membrane protein, integral; EC 2.7.10.1; Protein kinase, tyrosine (receptor); TK group; DDR family

Cellular Component: focal adhesion; membrane; integral to plasma membrane; apical plasma membrane; integral to membrane; plasma membrane

Molecular Function: transferase activity; collagen binding; protein-tyrosine kinase activity; transferase activity, transferring phosphorus-containing groups; nucleotide binding; transmembrane receptor protein tyrosine kinase activity; kinase activity; ATP binding; protein kinase activity

Biological Process: ossification; collagen fibril organization; peptidyl-tyrosine phosphorylation; protein amino acid autophosphorylation; protein amino acid phosphorylation; positive regulation of osteoblast differentiation; positive regulation of fibroblast proliferation; biomineral formation; positive regulation of protein kinase activity; positive regulation of cell proliferation; positive regulation of transcription factor activity; phosphorylation; regulation of bone mineralization; transmembrane receptor protein tyrosine kinase signaling pathway