DAPK3 sirna

DAPK3 siRNA (Mouse)

Cat. Num. AAA8207044

• Gene Names: Dapk3; dlk; ZIPK
• Reactivity: Mouse
• Applications: RNA Interference (RNAi)
• Purity: > 97%
• Synonyms: DAPK3; DAPK3 siRNA (Mouse); ZIPK; Death-associated protein kinase 3; DAP kinase 3; DAP-like kinase; Dlk; MYPT1 kinase; ZIP-kinase; DAPK3 sirna

• Host: Synthetic
• Reactivity: Mouse
• Specificity: DAPK3 siRNA (Mouse) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
• Purity/Purification: > 97%
• Form/Format: Lyophilized powder
• Sequence Length: 448

• Applicable Applications for DAPK3 sirna: RNA Interference (RNAi)
• Quality Control: Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
• Directions for Use: We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
• Components: We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of mouse DAPK3 gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
• Preparation and Storage: Shipped at 4 degree C. Store at -20 degree C for one year.

Related Product Information for DAPK3 sirna

siRNA to inhibit DAPK3 expression using RNA interference

NCBI and Uniprot Product Information

• NCBI GI #: 299758494
• NCBI GeneID: 13144
• NCBI Accession #: NP_001177402.1
• NCBI GenBank Nucleotide #: NM_001190473.1
• UniProt Accession #: O54784
• Molecular Weight: 51,422 Da
• NCBI Official Full Name: death-associated protein kinase 3 isoform b
• NCBI Official Synonym Full Names: death-associated protein kinase 3
• NCBI Official Symbol: Dapk3
• NCBI Official Synonym Symbols: dlk; ZIPK
• NCBI Protein Information: death-associated protein kinase 3
• UniProt Protein Name: Death-associated protein kinase 3
• Protein Family: Death-associated protein kinase
• UniProt Gene Name: Dapk3
• UniProt Synonym Gene Names: Zipk; DAP kinase 3; Dlk
• UniProt Entry Name: DAPK3_MOUSE

Uniprot Description

DAPK3: Serine/threonine kinase which is involved in the regulation of apoptosis, autophagy, transcription, actin cytoskeleton reorganization, cell motility, smooth muscle contraction, and mitosis, particularly cytokinesis. Regulates both type I apoptotic and type II autophagic cell deaths signal, depending on the cellular setting. The former is caspase- dependent, while the latter is caspase-independent and is characterized by the accumulation of autophagic vesicles. Regulates myosin phosphorylation in both smooth muscle and non- muscle cells. In smooth muscle, regulates myosin either directly by phosphorylating MYL12B and MYL9 or through inhibition of smooth muscle myosin phosphatase (SMPP1M) via phosphorylation of PPP1R12A, and the inhibition of SMPP1M functions to enhance muscle responsiveness to Ca(2+) and promote a contractile state. Enhances transcription from AR-responsive promoters in a hormone- and kinase-dependent manner. Phosphorylates STAT3 and enhances its transcriptional activity. Positively regulates the canonical Wnt/beta-catenin signaling through interaction with NLK and TCF7L2. Can disrupt the NLK-TCF7L2 complex thereby influencing the phosphorylation of TCF7L2 by NLK. Phosphorylates histone H3 on 'Thr-11' at centromeres during mitosis. Involved in the formation of promyelocytic leukemia protein nuclear body (PML-NB), one of many subnuclear domains in the eukaryotic cell nucleus, and which is involved in oncogenesis and viral infection. Monomer and homotrimer. Can also exist as homodimer or form heterodimers with ATF4. Homodimerization is required for activation segment autophosphorylation Both interactions require an intact leucine zipper domain and oligomerization is required for full enzymatic activity. Also binds to DAXX and PAWR, possibly in a ternary complex which plays a role in caspase activation. According to PubMed:17953487, does not interact with PARW. Interacts with AATF, CDC5L, UBE2D1, UBE2D2 AND UBE2D3. Interacts with AR and this interaction is enhanced by AATF. Interacts (via leucine zipper) with TCP10L (via leucine zipper). Interacts (via kinase domain) with DAPK1 (via kinase domain).Interacts with STAT3, NLK and TCF7L2. Isoform 1 and isoform 2 can interact with myosin and PPP1R12A. Isoform 2 is expressed in the bladder smooth muscle. Inhibited by pyridone 6 (K00225), a potent, ATP-competitive inhibitor. Phosphorylation at Thr-180, Thr-225 and Thr-265 is essential for activity. Oligomerization is required for full enzymatic activity. Belongs to the protein kinase superfamily. CAMK Ser/Thr protein kinase family. DAP kinase subfamily. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Kinase, protein; EC 2.7.11.1; Protein kinase, CAMK; Tumor suppressor; Protein kinase, Ser/Thr (non-receptor); CAMK group; DAPK family

Cellular Component: PML body; cytoskeleton; cytoplasm; chromosome; nucleus; chromosome, pericentric region; lipid raft

Molecular Function: identical protein binding; Rho GTPase binding; protein homodimerization activity; calmodulin-dependent protein kinase activity; nucleotide binding; protein kinase activity; transferase activity; protein serine/threonine kinase activity; protein binding; leucine zipper domain binding; transferase activity, transferring phosphorus-containing groups; kinase activity; ATP binding

Biological Process: transcription, DNA-dependent; apoptosis; positive regulation of apoptosis; protein amino acid autophosphorylation; regulation of mitotic cell cycle; regulation of myosin II filament assembly or disassembly; chromatin modification; protein amino acid phosphorylation; regulation of apoptosis; regulation of cell shape; regulation of transcription, DNA-dependent; negative regulation of translation; regulation of focal adhesion formation; phosphorylation; positive regulation of cell migration