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Western Blot (WB) (Western blot analysis of Glucocorticoid Receptor expression in Jurkat (A) whole cell lysates.)

Rabbit JUND Polyclonal Antibody | anti-JUND antibody

Anti-JUND Antibody

Gene Names
JUND; AP-1
Reactivity
Human, Mouse, Rat, Bovine, Chicken
Applications
Western Blot, Immunohistochemistry, Immunoprecipitation, Gel Super Shift Assay
Purity
The antibody was purified by immunogen affinity chromatography.
Synonyms
JUND; Polyclonal Antibody; Anti-JUND Antibody; Transcription factor jun-D; anti-JUND antibody
Ordering
For Research Use Only!
Host
Rabbit
Reactivity
Human, Mouse, Rat, Bovine, Chicken
Clonality
Polyclonal
Specificity
Recognizes endogenous levels of JUND protein.
Purity/Purification
The antibody was purified by immunogen affinity chromatography.
Form/Format
Liquid in 0.42% Potassium Phosphate, 0.87% Sodium Chloride, pH 7.3, 30% Glycerol, and 0.01% Sodium Azide.
Sequence Length
347
Applicable Applications for anti-JUND antibody
Western Blot (WB), Immunohistochemistry (IHC), Immunoprecipitation (IP), Electrophoretic Mobility Shift Assay (EMSA)
Application Notes
Western Blot: (1/500 - 1/1000); Immunohistochemistry: (1/100 - 1/200); Immunoprecipitation: (1/10 - 1/100)
Immunogen
KLH-conjugated synthetic peptide encompassing a sequence within the C-term region of human JUND. The exact sequence is proprietary.
Preparation and Storage
Shipped at 4 degree C. Upon delivery aliquot and store at -20 degree C for one year. Avoid freeze/thaw cycles.

Western Blot (WB)

(Western blot analysis of Glucocorticoid Receptor expression in Jurkat (A) whole cell lysates.)

Western Blot (WB) (Western blot analysis of Glucocorticoid Receptor expression in Jurkat (A) whole cell lysates.)

Immunohistochemistry (IHC)

(Immunohistochemical analysis of Glucocorticoid Receptor staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.)

Immunohistochemistry (IHC) (Immunohistochemical analysis of Glucocorticoid Receptor staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.)
Related Product Information for anti-JUND antibody
Rabbit polyclonal antibody to JUND

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
UniProt Accession #
Molecular Weight
35,174 Da
NCBI Official Full Name
Transcription factor jun-D
NCBI Official Synonym Full Names
jun D proto-oncogene
NCBI Official Symbol
JUND
NCBI Official Synonym Symbols
AP-1
NCBI Protein Information
transcription factor jun-D
UniProt Protein Name
Transcription factor jun-D
Protein Family
UniProt Gene Name
JUND
UniProt Entry Name
JUND_HUMAN

NCBI Description

The protein encoded by this intronless gene is a member of the JUN family, and a functional component of the AP1 transcription factor complex. This protein has been proposed to protect cells from p53-dependent senescence and apoptosis. Alternative translation initiation site usage results in the production of different isoforms (PMID:12105216). [provided by RefSeq, Nov 2013]

Uniprot Description

JunD: Transcription factor binding AP-1 sites. Binds DNA as a dimer. Interacts with MEN1; this interaction represses transcriptional activation. Belongs to the bZIP family. Jun subfamily.

Protein type: Transcription factor; Motility/polarity/chemotaxis

Chromosomal Location of Human Ortholog: 19p13.2

Cellular Component: chromatin; nuclear chromatin; nucleus; transcription factor complex

Molecular Function: enzyme binding; ligand-dependent nuclear receptor binding; protein binding; transcription coactivator activity; transcription factor binding

Biological Process: aging; cellular response to hormone stimulus; circadian rhythm; negative regulation of transcription from RNA polymerase II promoter; osteoblast development; positive regulation of cell differentiation; positive regulation of osteoblast differentiation; positive regulation of transcription from RNA polymerase II promoter; regulation of cell cycle; regulation of cell proliferation; regulation of transcription from RNA polymerase II promoter; response to cAMP; response to cytokine stimulus; response to drug; response to light stimulus; response to lipopolysaccharide; response to mechanical stimulus; response to peptide hormone stimulus; response to radiation; transcription from RNA polymerase II promoter

Research Articles on JUND

Similar Products

Product Notes

The JUND jund (Catalog #AAA8241999) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Anti-JUND Antibody reacts with Human, Mouse, Rat, Bovine, Chicken and may cross-react with other species as described in the data sheet. AAA Biotech's JUND can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunohistochemistry (IHC), Immunoprecipitation (IP), Electrophoretic Mobility Shift Assay (EMSA). Western Blot: (1/500 - 1/1000); Immunohistochemistry: (1/100 - 1/200); Immunoprecipitation: (1/10 - 1/100). Researchers should empirically determine the suitability of the JUND jund for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "JUND, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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