CSRP3 sirna

CSRP3 siRNA (Mouse)

Cat. Num. AAA8202758

• Gene Names: Csrp3; MLP; CRP3; MMLP
• Reactivity: Mouse
• Applications: RNA Interference (RNAi)
• Purity: > 97%
• Synonyms: CSRP3; CSRP3 siRNA (Mouse); CLP; MLP; Cysteine and glycine-rich protein 3; Cysteine-rich protein 3; CRP3; LIM domain protein. cardiac; Muscle LIM protein; CSRP3 sirna

• Host: Synthetic
• Reactivity: Mouse
• Specificity: CSRP3 siRNA (Mouse) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
• Purity/Purification: > 97%
• Form/Format: Lyophilized powder
• Sequence Length: 194

• Applicable Applications for CSRP3 sirna: RNA Interference (RNAi)
• Quality Control: Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
• Directions for Use: We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
• Components: We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of mouse CSRP3 gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
• Preparation and Storage: Shipped at 4 degree C. Store at -20 degree C for one year.

Related Product Information for CSRP3 sirna

siRNA to inhibit CSRP3 expression using RNA interference

NCBI and Uniprot Product Information

• NCBI GI #: 311771533
• NCBI GeneID: 13009
• NCBI Accession #: NP_001185770.1
• NCBI GenBank Nucleotide #: NM_001198841.1
• UniProt Accession #: P50462
• Molecular Weight: 20,895 Da
• NCBI Official Full Name: cysteine and glycine-rich protein 3
• NCBI Official Synonym Full Names: cysteine and glycine-rich protein 3
• NCBI Official Symbol: Csrp3
• NCBI Official Synonym Symbols: MLP; CRP3; MMLP
• NCBI Protein Information: cysteine and glycine-rich protein 3
• UniProt Protein Name: Cysteine and glycine-rich protein 3
• Protein Family: Cysteine and glycine-rich protein
• UniProt Gene Name: Csrp3
• UniProt Synonym Gene Names: Clp; Mlp; CRP3
• UniProt Entry Name: CSRP3_MOUSE

Uniprot Description

CSRP3: Positive regulator of myogenesis. Plays a crucial and specific role in the organization of cytosolic structures in cardiomyocytes. Could play a role in mechanical stretch sensing. May be a scaffold protein that promotes the assembly of interacting proteins at Z-line structures. It is essential for calcineurin anchorage to the Z line. Required for stress-induced calcineurin-NFAT activation. Defects in CSRP3 are the cause of cardiomyopathy dilated type 1M (CMD1M). Dilated cardiomyopathy is a disorder characterized by ventricular dilation and impaired systolic function, resulting in congestive heart failure and arrhythmia. Patients are at risk of premature death. Defects in CSRP3 are the cause of familial hypertrophic cardiomyopathy type 12 (CMH12). Familial hypertrophic cardiomyopathy is a hereditary heart disorder characterized by ventricular hypertrophy, which is usually asymmetric and often involves the interventricular septum. The symptoms include dyspnea, syncope, collapse, palpitations, and chest pain. They can be readily provoked by exercise. The disorder has inter- and intrafamilial variability ranging from benign to malignant forms with high risk of cardiac failure and sudden cardiac death.

Protein type: Transcription regulation

Cellular Component: cytoskeleton; cell; cytoplasm; nucleus; Z disc

Molecular Function: actinin binding; MRF binding; protein binding; telethonin binding; zinc ion binding; structural constituent of muscle; metal ion binding

Biological Process: cellular calcium ion homeostasis; cardiac muscle development; muscle development; protein localization in organelle; multicellular organismal development; positive regulation of transcription factor activity; cardiac myofibril assembly; positive regulation of transcription from RNA polymerase II promoter; cell differentiation; regulation of the force of heart contraction; cardiac muscle contraction