COL8A2 sirna

COL8A2 siRNA (Mouse)

Cat. Num. AAA8227628

• Gene Names: Col8a2; AI429819
• Reactivity: Mouse
• Applications: RNA Interference (RNAi)
• Purity: > 97%
• Synonyms: COL8A2; COL8A2 siRNA (Mouse); Collagen alpha-2(VIII) chain; Endothelial collagen; COL8A2 sirna

• Host: Synthetic
• Reactivity: Mouse
• Specificity: COL8A2 siRNA (Mouse) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
• Purity/Purification: > 97%
• Form/Format: Lyophilized powder
• Sequence Length: 643

• Applicable Applications for COL8A2 sirna: RNA Interference (RNAi)
• Quality Control: Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
• Directions for Use: We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
• Components: We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of mouse COL8A2 gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
• Preparation and Storage: Shipped at 4 degree C. Store at -20 degree C for one year.

Related Product Information for COL8A2 sirna

siRNA to inhibit COL8A2 expression using RNA interference

NCBI and Uniprot Product Information

• NCBI GI #: 41054950
• NCBI GeneID: 329941
• NCBI Accession #: NP_955767.1
• NCBI GenBank Nucleotide #: NM_199473.2
• UniProt Accession #: P25318; Q68ED0; Q6KAQ4; Q6P1C4; A3KFY2
• Molecular Weight: 61,831 Da
• NCBI Official Full Name: collagen alpha-2(VIII) chain
• NCBI Official Synonym Full Names: collagen, type VIII, alpha 2
• NCBI Official Symbol: Col8a2
• NCBI Official Synonym Symbols: AI429819
• NCBI Protein Information: collagen alpha-2(VIII) chain
• UniProt Protein Name: Collagen alpha-2(VIII) chain
• UniProt Gene Name: Col8a2
• UniProt Entry Name: CO8A2_MOUSE

Uniprot Description

COL8A2: Macromolecular component of the subendothelium. Major component of the Descemet's membrane (basement membrane) of corneal endothelial cells. Also component of the endothelia of blood vessels. Necessary for migration and proliferation of vascular smooth muscle cells and thus, has a potential role in the maintenance of vessel wall integrity and structure, in particular in atherogenesis. Defects in COL8A2 are the cause of corneal dystrophy Fuchs endothelial type 1 (FECD1). It is an ocular disorder caused by loss of endothelium of the central cornea. It is characterized by focal wart-like guttata that arise from Descemet membrane and develop in the central cornea, epithelial blisters, reduced vision and pain. Descemet membrane is thickened by abnormal collagenous deposition. Defects in COL8A2 are the cause of posterior polymorphous corneal dystrophy type 2 (PPCD2). PPCD is a rare bilateral familial disorder of the corneal epithelium, and is inherited in a autosomal dominant pattern. The clinical features usually present earlier than FECD, being from birth onwards. The disorder is characterized by alterations of Descemet membrane presenting as vesicles, opacities or band-like lesions on slit- lamp examination and specular microscopy. Affected patient typically are asymptomatic.

Protein type: Secreted, signal peptide; Secreted; Extracellular matrix

Cellular Component: extracellular matrix; proteinaceous extracellular matrix; collagen; extracellular region; basement membrane

Biological Process: epithelial cell proliferation; camera-type eye morphogenesis; angiogenesis; cell adhesion