CHEK2 sirna

CHEK2 siRNA (Mouse)

Cat. Num. AAA8205951

• Gene Names: Chek2; CHK2; Cds1; Rad53; HUCDS1
• Reactivity: Mouse
• Applications: RNA Interference (RNAi)
• Purity: > 97%
• Synonyms: CHEK2; CHEK2 siRNA (Mouse); CHK2; RAD53; Serine/threonine-protein kinase Chk2; CHK2 checkpoint homolog; Checkpoint kinase 2; CHEK2 sirna

• Host: Synthetic
• Reactivity: Mouse
• Specificity: CHEK2 siRNA (Mouse) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
• Purity/Purification: > 97%
• Form/Format: Lyophilized powder
• Sequence Length: 546

• Applicable Applications for CHEK2 sirna: RNA Interference (RNAi)
• Quality Control: Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
• Directions for Use: We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
• Components: We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of mouse CHEK2 gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
• Preparation and Storage: Shipped at 4 degree C. Store at -20 degree C for one year.

Related Product Information for CHEK2 sirna

siRNA to inhibit CHEK2 expression using RNA interference

NCBI and Uniprot Product Information

• NCBI GI #: 7710088
• NCBI GeneID: 50883
• NCBI Accession #: NP_057890.1
• NCBI GenBank Nucleotide #: NM_016681.3
• UniProt Accession #: Q9Z265
• Molecular Weight: 61,088 Da
• NCBI Official Full Name: serine/threonine-protein kinase Chk2
• NCBI Official Synonym Full Names: checkpoint kinase 2
• NCBI Official Symbol: Chek2
• NCBI Official Synonym Symbols: CHK2; Cds1; Rad53; HUCDS1
• NCBI Protein Information: serine/threonine-protein kinase Chk2
• UniProt Protein Name: Serine/threonine-protein kinase Chk2
• Protein Family: Serine/threonine-protein kinase
• UniProt Gene Name: Chek2
• UniProt Synonym Gene Names: Chk2; Rad53
• UniProt Entry Name: CHK2_MOUSE

Uniprot Description

Chk2: a CAMK protein kinase of the Rad53 family. A cell cycle checkpoint regulator and putative tumor suppressor. It contains a forkhead-associated protein interaction domain essential for activation in response to DNA damage and is rapidly phosphorylated in response to replication blocks and DNA damage. Phosphorylates and inhibits CDC25C, preventing entry into mitosis, p53, leading to cell cycle arrest in G1, and BRCA1, restoring survival after DNA damage. Twelve splice variant isoforms have been described for human Chk2. LOF mutants cause Li-Fraumeni syndrome, a highly penetrant familial cancer phenotype also caused by p53 mutations. Familial mutations also associated with prostate and breast cancer, and mutations also seen in a variety of sporadic cancers and cell lines.

Protein type: Protein kinase, Ser/Thr (non-receptor); Protein kinase, CAMK; Kinase, protein; EC 2.7.11.1; Tumor suppressor; CAMK group; RAD53 family

Cellular Component: nucleoplasm; Golgi apparatus; chromosome, telomeric region; PML body; intracellular; nucleus

Molecular Function: transferase activity; protein serine/threonine kinase activity; identical protein binding; protein homodimerization activity; metal ion binding; ubiquitin protein ligase binding; transferase activity, transferring phosphorus-containing groups; nucleotide binding; kinase activity; protein kinase binding; ATP binding; protein kinase activity

Biological Process: mitosis; transcription, DNA-dependent; DNA damage induced protein phosphorylation; protein stabilization; apoptosis; positive regulation of transcription, DNA-dependent; protein amino acid autophosphorylation; DNA damage response, signal transduction; cell cycle; DNA repair; protein amino acid phosphorylation; regulation of transcription, DNA-dependent; DNA damage response, signal transduction resulting in induction of apoptosis; cellular protein catabolic process; cell division; double-strand break repair; DNA damage checkpoint; response to gamma radiation; regulation of protein catabolic process; G2/M transition of mitotic cell cycle; phosphorylation; response to DNA damage stimulus