Structure
(Fig-1: chemical structure of CpG ODN)
ODN ligand
CpG ODN (1668), TLR9 ligand (Class B)
Reactivity
Rat, Mouse
Applications
Functional Assay
Synonyms
ODN; CpG ODN (1668); TLR9 ligand (Class B); ODN ligand
Reactivity
Rat, Mouse
Form/Format
1 mg/ml in entotoxin-free water
Applicable Applications for ODN ligand
Functional Assay
Application Notes
A. CpG ODN 1668-mediated mouse TLR9 activation in NF-kBLeeporter; RAW 264.7 cells (Figure 2). 1. Harvest NF-kB Leeporter; RAW 264.7 cells and seed cells into a white solid-bottom 96-well microplate in 100 ul of growth medium at 5 x 104cells/well. 2. Incubate cells at 37 degree C in a CO2incubator for overnight. 3. The next day, stimulate cells with various amounts of CpG ODN 1668. 4. Incubate at 37 degree C in a CO2incubator for 6-16 hours. 5. Add 30-50 ul of luciferase assay reagent per well. 6. Incubate at room temperature for 1-5 minutes and measure luminescence using a microplate luminometer.
Preparation and Storage
Upon receipt, store at -20 degree C (Stable for at least 6 months).
Avoid frequent freeze/thaw cycles.
Avoid frequent freeze/thaw cycles.
Structure
(Fig-1: chemical structure of CpG ODN)
Structure
(Fig-1: chemical structure of CpG ODN)
Testing Data
(Fig-2. Induction of mouse TLR9 activity by CpG ODN 1668.)
Testing Data
(Fig-2. Induction of mouse TLR9 activity by CpG ODN 1668.)
Related Product Information for ODN ligand
Sequence: 5'-tccatgaCGttcctgatgct-3' (Class B ODN 1668: Bases are phosphorothioate.) Synthetic oligodeoxynucleotides (ODN) containing unmethylated deoxycytosine-deoxyguanosine (CpG) motif are equivalent to bacterial DNA in the immunostimulatory activity, which can induce innate immunity via Toll-like receptor 9 (TLR9) in mammals. There are three major classes of CpG ODNs, and each ODN class exhibits different stimulatory effects on immune cell activation. Class A ODNs are potent inducers of IFN-alpha that leads to the plasmacytoid dendritc cell (pDC) maturation. Class B ODNs are relatively weak inducers of type I IFNs but strong stimulators of human B cells and monocyte maturation. Class C ODNs combine elements of both Classes A and B ODNs, which can induce IFN-alpha in pDC and activation of B cells.
Product Categories/Family for ODN ligand
Similar Products
Product Notes
The ODN (Catalog #AAA668910) is a Ligand and is intended for research purposes only. The product is available for immediate purchase. The CpG ODN (1668), TLR9 ligand (Class B) reacts with Rat, Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's ODN can be used in a range of immunoassay formats including, but not limited to, Functional Assay. A. CpG ODN 1668-mediated mouse TLR9 activation in NF-kBLeeporter; RAW 264.7 cells (Figure 2). 1. Harvest NF-kB Leeporter; RAW 264.7 cells and seed cells into a white solid-bottom 96-well microplate in 100 ul of growth medium at 5 x 104cells/well. 2. Incubate cells at 37 degree C in a CO2incubator for overnight. 3. The next day, stimulate cells with various amounts of CpG ODN 1668. 4. Incubate at 37 degree C in a CO2incubator for 6-16 hours. 5. Add 30-50 ul of luciferase assay reagent per well. 6. Incubate at room temperature for 1-5 minutes and measure luminescence using a microplate luminometer. Researchers should empirically determine the suitability of the ODN for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "ODN, Ligand" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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