CHD7 sirna

CHD7 siRNA (Mouse)

Cat. Num. AAA8217657

• Gene Names: Chd7; Dz; Mt; Cyn; Edy; Flo; Lda; Obt; Whi; Cycn; Todo; WBE1; metis; GENA 47; GENA 60; Gena 52; A730019I05Rik
• Reactivity: Mouse
• Applications: RNA Interference (RNAi)
• Purity: > 97%
• Synonyms: CHD7; CHD7 siRNA (Mouse); Chromodomain-helicase-DNA-binding protein 7; CHD-7; ATP-dependent helicase CHD7; CHD7 sirna

• Host: Synthetic
• Reactivity: Mouse
• Specificity: CHD7 siRNA (Mouse) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
• Purity/Purification: > 97%
• Form/Format: Lyophilized powder
• Sequence Length: 2,960

• Applicable Applications for CHD7 sirna: RNA Interference (RNAi)
• Quality Control: Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
• Directions for Use: We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
• Components: We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of mouse CHD7 gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
• Preparation and Storage: Shipped at 4 degree C. Store at -20 degree C for one year.

Related Product Information for CHD7 sirna

siRNA to inhibit CHD7 expression using RNA interference

NCBI and Uniprot Product Information

• NCBI GI #: 460838688
• NCBI GeneID: 320790
• NCBI Accession #: NP_001264078.1
• NCBI GenBank Nucleotide #: NM_001277149.1
• UniProt Accession #: A2AJK6; Q3TA86; Q3TAG7; Q3TBU4; Q8C986; Q8K244
• Molecular Weight: 331,195 Da
• NCBI Official Full Name: chromodomain-helicase-DNA-binding protein 7
• NCBI Official Synonym Full Names: chromodomain helicase DNA binding protein 7
• NCBI Official Symbol: Chd7
• NCBI Official Synonym Symbols: Dz; Mt; Cyn; Edy; Flo; Lda; Obt; Whi; Cycn; Todo; WBE1; metis; GENA 47; GENA 60; Gena 52; A730019I05Rik
• NCBI Protein Information: chromodomain-helicase-DNA-binding protein 7
• UniProt Protein Name: Chromodomain-helicase-DNA-binding protein 7
• Protein Family: Chromodomain-helicase-DNA-binding protein
• UniProt Gene Name: Chd7
• UniProt Synonym Gene Names: CHD-7
• UniProt Entry Name: CHD7_MOUSE

NCBI Description

This gene encodes a protein containing two chromodomains and an ATP-binding helicase domain that functions as a regulator of transcription. Mutations in this gene result in an array of development defects, including inner ear problems. The related gene in humans has been implicated in CHARGE syndrome. [provided by RefSeq, Mar 2013]

Uniprot Description

CHD-7: Probable transcription regulator. May interact with CTCF. Interacts with CHD8. Widely expressed in fetal and adult tissues. Belongs to the SNF2/RAD54 helicase family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: DNA-binding; EC 3.6.4.12; Nucleolus; Helicase

Cellular Component: nucleoplasm; nucleus

Molecular Function: protein binding; hydrolase activity, acting on acid anhydrides; DNA binding; hydrolase activity; nucleotide binding; chromatin binding; helicase activity; ATP binding

Biological Process: heart morphogenesis; limb development; central nervous system development; olfactory behavior; blood circulation; locomotory behavior; palate development; ear morphogenesis; positive regulation of multicellular organism growth; female genitalia development; adult walking behavior; embryonic hindlimb morphogenesis; regulation of growth hormone secretion; olfactory nerve development; sensory perception of sound; regulation of neurogenesis; regulation of transcription, DNA-dependent; skeletal development; T cell differentiation; adult heart development; blood vessel development; inner ear morphogenesis; camera-type eye development; transcription, DNA-dependent; in utero embryonic development; olfactory bulb development; semicircular canal morphogenesis; cranial nerve development; chromatin modification; genitalia development; retina development in camera-type eye; artery morphogenesis; cognition; nose development; rRNA processing