CFD sirna

CFD siRNA (Mouse)

Cat. Num. AAA8221132

• Gene Names: Cfd; DF; Adn
• Reactivity: Mouse
• Applications: RNA Interference (RNAi)
• Purity: > 97%
• Synonyms: CFD; CFD siRNA (Mouse); ADN; DF; Complement factor D; 28 kDa adipocyte protein; Adipsin; C3 convertase activator; Properdin factor D; CFD sirna

• Host: Synthetic
• Reactivity: Mouse
• Specificity: CFD siRNA (Mouse) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
• Purity/Purification: > 97%
• Form/Format: Lyophilized powder
• Sequence Length: 258

• Applicable Applications for CFD sirna: RNA Interference (RNAi)
• Quality Control: Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
• Directions for Use: We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
• Components: We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of mouse CFD gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
• Preparation and Storage: Shipped at 4 degree C. Store at -20 degree C for one year.

Related Product Information for CFD sirna

siRNA to inhibit CFD expression using RNA interference

NCBI and Uniprot Product Information

• NCBI GI #: 632794820
• NCBI GeneID: 11537
• NCBI Accession #: NP_001278844.1
• NCBI GenBank Nucleotide #: NM_001291915.1
• UniProt Accession #: P03953; Q61280
• Molecular Weight: 27,986 Da
• NCBI Official Full Name: complement factor D isoform 2
• NCBI Official Synonym Full Names: complement factor D (adipsin)
• NCBI Official Symbol: Cfd
• NCBI Official Synonym Symbols: DF; Adn
• NCBI Protein Information: complement factor D
• UniProt Protein Name: Complement factor D
• Protein Family: Complement factor
• UniProt Gene Name: Cfd
• UniProt Synonym Gene Names: Adn; Df
• UniProt Entry Name: CFAD_MOUSE

Uniprot Description

CFD: Factor D cleaves factor B when the latter is complexed with factor C3b, activating the C3bbb complex, which then becomes the C3 convertase of the alternate pathway. Its function is homologous to that of C1s in the classical pathway. Defects in CFD are the cause of complement factor D deficiency (CFDD). CFDD is an immunologic disorder characterized by increased susceptibility to bacterial infections, particularly Neisseria infections, due to a defect in the alternative complement pathway. Belongs to the peptidase S1 family.

Protein type: Protease; Secreted, signal peptide; Secreted; EC 3.4.21.46

Cellular Component: extracellular space; extracellular region

Molecular Function: peptidase activity; hydrolase activity; serine-type peptidase activity; serine-type endopeptidase activity; endopeptidase activity; catalytic activity

Biological Process: Notch signaling pathway; immune system process; complement activation, alternative pathway; innate immune response; proteolysis