CDKN2C sirna

CDKN2C siRNA (Mouse)

Cat. Num. AAA8231508

• Gene Names: Cdkn2c; p18; INK4c; C77269; p18-INK6; p18INK4c; p18-INK4c
• Reactivity: Mouse
• Applications: RNA Interference (RNAi)
• Purity: > 97%
• Synonyms: CDKN2C; CDKN2C siRNA (Mouse); Cyclin-dependent kinase 4 inhibitor C; Cyclin-dependent kinase 6 inhibitor; p18-INK4c; p18-INK6; CDKN2C sirna

• Host: Synthetic
• Reactivity: Mouse
• Specificity: CDKN2C siRNA (Mouse) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
• Purity/Purification: > 97%
• Form/Format: Lyophilized powder
• Sequence Length: 168

• Applicable Applications for CDKN2C sirna: RNA Interference (RNAi)
• Quality Control: Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
• Directions for Use: We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
• Components: We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of mouse CDKN2C gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
• Preparation and Storage: Shipped at 4 degree C. Store at -20 degree C for one year.

Related Product Information for CDKN2C sirna

siRNA to inhibit CDKN2C expression using RNA interference

NCBI and Uniprot Product Information

• NCBI GI #: 672717917
• NCBI GeneID: 12580
• NCBI Accession #: NP_001288297.1
• NCBI GenBank Nucleotide #: NM_001301368.1
• UniProt Accession #: Q60772
• Molecular Weight: 18,066 Da
• NCBI Official Full Name: cyclin-dependent kinase 4 inhibitor C
• NCBI Official Synonym Full Names: cyclin-dependent kinase inhibitor 2C (p18, inhibits CDK4)
• NCBI Official Symbol: Cdkn2c
• NCBI Official Synonym Symbols: p18; INK4c; C77269; p18-INK6; p18INK4c; p18-INK4c
• NCBI Protein Information: cyclin-dependent kinase 4 inhibitor C
• UniProt Protein Name: Cyclin-dependent kinase 4 inhibitor C
• Protein Family: Cyclin-dependent kinase 4 inhibitor
• UniProt Gene Name: Cdkn2c
• UniProt Entry Name: CDN2C_MOUSE

NCBI Description

The protein encoded by this gene is a member of the INK4 family of cyclin-dependent kinase (cdk) inhibitors, and contains five ankyrin repeats. This protein interacts with both Cdk4 and Cdk6 to inhibit their kinase activities, and prevent their interactions with D-type cyclins, thereby negatively regulating cell division. This gene is differentially expressed in a variety of tissues, and is cell cycle regulated. Deletion of this gene can lead to tumor growth. Maximal expression is observed at the G2/M phase. Alternative splicing and promoter usage results in multiple transript variants. [provided by RefSeq, Aug 2014]

Uniprot Description

CDKN2C: Interacts strongly with CDK6, weakly with CDK4. Inhibits cell growth and proliferation with a correlated dependence on endogenous retinoblastoma protein RB. Heterodimer of p18 with CDK6. Highest levels found in skeletal muscle. Also found in pancreas and heart. Belongs to the CDKN2 cyclin-dependent kinase inhibitor family.

Protein type: Inhibitor; Protein kinase, regulatory subunit; Cell cycle regulation

Cellular Component: cytoplasm; nucleus

Molecular Function: cyclin-dependent protein kinase inhibitor activity; protein kinase binding

Biological Process: negative regulation of cell proliferation; negative regulation of phosphorylation; negative regulation of cell growth; regulation of cyclin-dependent protein kinase activity; cell cycle arrest; cell cycle; G1/S transition of mitotic cell cycle