Rabbit anti-Human FANCB Polyclonal Antibody | anti-FANCB antibody

FANCB, ID (FANCB, Fanconi anemia group B protein, Fanconi anemia-associated polypeptide of 95kD) (Azide free) (HRP)

Cat. Num. AAA6297981

• Gene Names: FANCB; FA2; FAB; FACB; FAAP90; FAAP95
• Reactivity: Human
• Applications: Western Blot, ELISA
• Purity: Purified by Protein A Affinity Chromatography.
• Synonyms: FANCB; Polyclonal Antibody; ID (FANCB; Fanconi anemia group B protein; Fanconi anemia-associated polypeptide of 95kD) (Azide free) (HRP); Fanconi anemia-associated polypeptide of 95kD; anti-FANCB antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Purified by Protein A Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Horseradish Peroxidase (HRP).

• Applicable Applications for anti-FANCB antibody: Western Blot (WB), ELISA (EIA)
• Application Notes: WB: 1:100-500; ELISA: 1:1,000; Applications are based on unconjugated antibody.
• Immunogen: FANCB antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 359-388 amino acids from the Central region of human FANCB.
• Conjugate: HRP
• Note: Preservative Free
• Preparation and Storage: Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Note: Sodium azide is a potent inhibitor of peroxidase and should not be added to HRP conjugates. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(FANCB Antibody (Center) western blot analysis in A549 cell line lysates (35ug/lane).This demonstrates the FANCB antibody detected the FANCB protein (arrow).)

Related Product Information for anti-FANCB antibody

The Fanconi anemia complementation group (FANC) currently includes FANCA, FANCB, FANCC, FANCD1 (also called BRCA2), FANCD2, FANCE, FANCF, FANCG, FANCI, FANCJ (also called BRIP1), FANCL, FANCM and FANCN (also called PALB2). The previously defined group FANCH is the same as FANCA. Fanconi anemia is a genetically heterogeneous recessive disorder characterized by cytogenetic instability, hypersensitivity to DNA crosslinking agents, increased chromosomal breakage, and defective DNA repair. The members of the Fanconi anemia complementation group do not share sequence similarity; they are related by their assembly into a common nuclear protein complex. This gene encodes the protein for complementation group B. Alternative splicing results in two transcript variants encoding the same protein.

Product Categories/Family for anti-FANCB antibody

Antibodies; Fanconi Proteins

References

Cheung, K.L., et al. World J Surg Oncol 8, 38 (2010): Smith, I.M., et al. ORL J. Otorhinolaryngol. Relat. Spec. 72(1):44-50(2010). Barroso, E., et al. Breast Cancer Res. Treat. 118(3):655-660(2009). Garcia, M.J., et al. Breast Cancer Res. Treat. 113(3):545-551(2009). Nomura, Y., et al. Genes Cells 12(10):1111-1122(2007).

NCBI and Uniprot Product Information

• NCBI GI #: 66528785
• NCBI GeneID: 2187
• NCBI Accession #: NP_001018123.1
• NCBI GenBank Nucleotide #: NM_001018113.1
• UniProt Accession #: Q8NB91; Q7Z2U2; Q86XG1; B2RMZ4
• Molecular Weight: 97,726 Da
• NCBI Official Full Name: Fanconi anemia group B protein
• NCBI Official Synonym Full Names: Fanconi anemia, complementation group B
• NCBI Official Symbol: FANCB
• NCBI Official Synonym Symbols: FA2; FAB; FACB; FAAP90; FAAP95
• NCBI Protein Information: Fanconi anemia group B protein; Fanconi anemia-associated polypeptide of 95 kDa
• UniProt Protein Name: Fanconi anemia group B protein
• Protein Family: Fanconi anemia group B protein
• UniProt Gene Name: FANCB
• UniProt Synonym Gene Names: Protein FACB; FAAP95
• UniProt Entry Name: FANCB_HUMAN

NCBI Description

The Fanconi anemia complementation group (FANC) currently includes FANCA, FANCB, FANCC, FANCD1 (also called BRCA2), FANCD2, FANCE, FANCF, FANCG, FANCI, FANCJ (also called BRIP1), FANCL, FANCM and FANCN (also called PALB2). The previously defined group FANCH is the same as FANCA. Fanconi anemia is a genetically heterogeneous recessive disorder characterized by cytogenetic instability, hypersensitivity to DNA crosslinking agents, increased chromosomal breakage, and defective DNA repair. The members of the Fanconi anemia complementation group do not share sequence similarity; they are related by their assembly into a common nuclear protein complex. This gene encodes the protein for complementation group B. Alternative splicing results in two transcript variants encoding the same protein. [provided by RefSeq, Jul 2008]

Uniprot Description

Function: DNA repair protein required for FANCD2 ubiquitination. Ref.4

Subunit structure: Belongs to the multisubunit FA complex composed of FANCA, FANCB, FANCC, FANCE, FANCF, FANCG, FANCL/PHF9 and FANCM. The complex is not found in FA patients. Ref.4 Ref.5

Subcellular location: Nucleus Ref.4.

Involvement in disease: Fanconi anemia complementation group B (FANCB) [MIM:300514]: A disorder affecting all bone marrow elements and resulting in anemia, leukopenia and thrombopenia. It is associated with cardiac, renal and limb malformations, dermal pigmentary changes, and a predisposition to the development of malignancies. At the cellular level it is associated with hypersensitivity to DNA-damaging agents, chromosomal instability (increased chromosome breakage) and defective DNA repair. Some severe FANCB cases manifest features of VACTERL syndrome with hydrocephalus.Note: The disease is caused by mutations affecting the gene represented in this entry. Ref.4 Ref.6

Sequence caution: The sequence AAH43596.1 differs from that shown. Reason: Contaminating sequence. Potential poly-A sequence.The sequence AAH55411.1 differs from that shown. Reason: Contaminating sequence. Potential poly-A sequence.