CDK12 sirna

CDK12 siRNA (Mouse)

Cat. Num. AAA8222881

• Gene Names: Cdk12; Crk7; Pksc; Crkrs; AI646528; D11Ertd752e; 1810022J16Rik
• Reactivity: Mouse
• Applications: RNA Interference (RNAi)
• Purity: > 97%
• Synonyms: CDK12; CDK12 siRNA (Mouse); CRK7; CRKRS; KIAA0904; Cyclin-dependent kinase 12; Cdc2-related kinase. arginine/serine-rich; CrkRS; Cell division cycle 2-related protein kinase 7; CDC2-related protein kinase 7; Cell division protein kinase 12; CDK12 sirna

• Host: Synthetic
• Reactivity: Mouse
• Specificity: CDK12 siRNA (Mouse) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
• Purity/Purification: > 97%
• Form/Format: Lyophilized powder
• Sequence Length: 1484

• Applicable Applications for CDK12 sirna: RNA Interference (RNAi)
• Quality Control: Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
• Directions for Use: We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
• Components: We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of mouse CDK12 gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
• Preparation and Storage: Shipped at 4 degree C. Store at -20 degree C for one year.

Related Product Information for CDK12 sirna

siRNA to inhibit CDK12 expression using RNA interference

NCBI and Uniprot Product Information

• NCBI GI #: 157816935
• NCBI GeneID: 69131
• NCBI Accession #: NP_001103096.1
• NCBI GenBank Nucleotide #: NM_001109626.1
• UniProt Accession #: Q14AX6; Q6ZQ27; Q8R457; A2A530; A2A531; B1AQH7
• Molecular Weight: 162,755 Da
• NCBI Official Full Name: cyclin-dependent kinase 12 isoform 1
• NCBI Official Synonym Full Names: cyclin-dependent kinase 12
• NCBI Official Symbol: Cdk12
• NCBI Official Synonym Symbols: Crk7; Pksc; Crkrs; AI646528; D11Ertd752e; 1810022J16Rik
• NCBI Protein Information: cyclin-dependent kinase 12
• UniProt Protein Name: Cyclin-dependent kinase 12
• Protein Family: Cyclin-dependent kinase
• UniProt Gene Name: Cdk12
• UniProt Synonym Gene Names: Crk7; Crkrs; Kiaa0904; CrkRS; CDC2-related protein kinase 7
• UniProt Entry Name: CDK12_MOUSE

Uniprot Description

CDK12: a Ser/Thr protein kinase of the CMGC group and CDK family. Phosphorylates the C-terminal heptapeptide repeat domain (CTD) of the largest RNA polymerase II subunit RPB1, thereby acting as a key regulator of transcription elongation. Required for RNA splicing, possibly by phosphorylating SRSF1/SF2. Interacts with CCNL1 and CCNL2. Colocalized with nuclear speckles throughout interphase. Has a highly phosphorylated arginine/serine-rich (RS) domain N-terminal to the kinase domain. Chromosomal aberrations involving CDK12 may be a cause gastric cancer. Deletions within 17q12 region producing fusion transcripts with ERBB2, leading to CDK12-ERBB2 fusion leading to trunctated CDK12 protein not in-frame with ERBB2. Three isoforms of the human protein are produced by alternative splicing.

Protein type: EC 2.7.11.22; Kinase, protein; Spliceosome; Protein kinase, CMGC; Motility/polarity/chemotaxis; Cell cycle regulation; EC 2.7.11.23; RNA splicing; Protein kinase, Ser/Thr (non-receptor); CMGC group; CDK family; CRK7 subfamily; CDK/CRK7 subfamily

Cellular Component: nucleoplasm; nuclear cyclin-dependent protein kinase holoenzyme complex; nucleolus; nuclear speck; nucleus

Molecular Function: transferase activity; RNA polymerase subunit kinase activity; protein serine/threonine kinase activity; cyclin binding; cyclin-dependent protein kinase activity; transferase activity, transferring phosphorus-containing groups; nucleotide binding; kinase activity; ATP binding; protein kinase activity

Biological Process: regulation of RNA splicing; protein amino acid autophosphorylation; RNA splicing; regulation of MAP kinase activity; mRNA processing; protein amino acid phosphorylation; phosphorylation