CD2AP sirna

CD2AP siRNA (Mouse)

Cat. Num. AAA8235838

• Gene Names: Cd2ap; Mets1; C78928; METS-1; AL024079
• Reactivity: Mouse
• Applications: RNA Interference (RNAi)
• Purity: > 97%
• Synonyms: CD2AP; CD2AP siRNA (Mouse); METS1; CD2-associated protein; Mesenchyme-to-epithelium transition protein with SH3 domains 1; METS-1; CD2AP sirna

• Host: Synthetic
• Reactivity: Mouse
• Specificity: CD2AP siRNA (Mouse) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
• Purity/Purification: > 97%
• Form/Format: Lyophilized powder
• Sequence Length: 637

• Applicable Applications for CD2AP sirna: RNA Interference (RNAi)
• Quality Control: Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
• Directions for Use: We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
• Components: We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of mouse CD2AP gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
• Preparation and Storage: Shipped at 4 degree C. Store at -20 degree C for one year.

Related Product Information for CD2AP sirna

siRNA to inhibit CD2AP expression using RNA interference

NCBI and Uniprot Product Information

• NCBI GI #: 125987599
• NCBI GeneID: 12488
• NCBI Accession #: NP_033977.3
• NCBI GenBank Nucleotide #: NM_009847.3
• UniProt Accession #: Q9JLQ0; O88903; Q8K4Z1; Q8VCI9; E9QL86
• Molecular Weight: 70,450 Da
• NCBI Official Full Name: CD2-associated protein
• NCBI Official Synonym Full Names: CD2-associated protein
• NCBI Official Symbol: Cd2ap
• NCBI Official Synonym Symbols: Mets1; C78928; METS-1; AL024079
• NCBI Protein Information: CD2-associated protein
• UniProt Protein Name: CD2-associated protein
• Protein Family: CD2-associated protein
• UniProt Gene Name: Cd2ap
• UniProt Synonym Gene Names: Mets1; METS-1
• UniProt Entry Name: CD2AP_MOUSE

NCBI Description

This gene encodes a scaffolding molecule that regulates the actin cytoskeleton. The protein directly interacts with filamentous actin and a variety of cell membrane proteins through multiple actin binding sites, SH3 domains, and a proline-rich region containing binding sites for SH3 domains. The cytoplasmic protein localizes to membrane ruffles, lipid rafts, and the leading edges of cells. It is implicated in dynamic actin remodeling and membrane trafficking that occurs during receptor endocytosis and cytokinesis. The mouse genome contains at least two pseudogenes located on chromosomes 9 and 17. [provided by RefSeq, Jul 2008]

Uniprot Description

CD2AP: an adapter protein between membrane proteins and the actin cytoskeleton. May play a role in receptor clustering and cytoskeletal polarity in the junction between T-cell and antigen-presenting cell. May anchor the podocyte slit diaphragm to the actin cytoskeleton in renal glomerolus. Required for cytokinesis. Links CD2 engagement to the WASp-evoked actin polymerization required for synapse formation and T cell activation. Attaches to membrane proteins via its three SH3 domains and attaches to and rearranges the cytoskeleton via its C-terminus. CD2AP?s interactions with the cytoskeleton result in receptor clustering and endocysosis as well as targeting proteins to degradative vesicles in the kidney. Mice carrying a heterozygous knockout of CD2AP show defects in kidney filtration, and mutations in the human gene are associated with focal segmental glomerulosclerosis (FSGS3).

Protein type: Adaptor/scaffold; Motility/polarity/chemotaxis

Cellular Component: protein complex; leading edge; intercellular junction; cell cortex; ruffle; filamentous actin; cytoskeleton; cell projection; endocytic vesicle; perinuclear region of cytoplasm; cytoplasm; nucleolus; plasma membrane

Molecular Function: protein C-terminus binding; protein binding; cadherin binding; beta-catenin binding; protein complex binding; vascular endothelial growth factor receptor binding; SH3 domain binding

Biological Process: mitosis; proteasomal ubiquitin-dependent protein catabolic process; cell migration; cell-cell adhesion; cell division; vesicle organization and biogenesis; cell cycle; regulation of receptor-mediated endocytosis; negative regulation of transforming growth factor-beta1 production