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Typical Testing Data/Standard Curve (for reference only) (Fig.1. Human Solute carrier family 15 member 1 (SLC15A1) Standard Curve.)

Human Solute Carrier Family 15 Member 1 (SLC15A1) ELISA Kit | SLC15A1 elisa kit

Human Solute Carrier Family 15 Member 1 (SLC15A1) ELISA Kit

Gene Names
SLC15A1; PEPT1; HPECT1; HPEPT1
Reactivity
Human
Synonyms
Solute Carrier Family 15 Member 1 (SLC15A1); Human Solute Carrier Family 15 Member 1 (SLC15A1) ELISA Kit; SLC15A1; RP11-130L10.1; HPECT1; HPEPT1; PEPT1; Caco-2 oligopeptide transporter; bA551M18.1.1 (solute carrier family 15 (oligopeptide transporter) member 1); intestinal H+/peptide cotransporter; macrophage oligo; SLC15A1 elisa kit
Ordering
For Research Use Only!
Reactivity
Human
Specificity
Human Solute carrier family 15 member 1 (SLC15A1) ELISA Kit has high sensitivity and excellent specificity for detection of Human SLC15A1. No significant cross-reactivity or interference between Human SLC15A1 and analogues was observed.
Sequence Length
708
Assay Type
Sandwich ELISA (Quantitative)
Samples
Cell Culture Supernatants, Serum, Plasma, Other Biological Fluids
Detection Method
Colorimetric
Usage Notes
* Do not mix components from different kit lots or use reagents beyond the kit expiration date.
* Allow all reagents to warm to room temperature for at least 30 minutes before opening.
* Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
* Unused wells must be kept desiccated at 4 degree C in the sealed bag provided.
* Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
* It is recommended that all samples and standards be assayed in duplicate or triplicate.
Preparation and Storage
Store at 2-8 degree C.

Typical Testing Data/Standard Curve (for reference only)

(Fig.1. Human Solute carrier family 15 member 1 (SLC15A1) Standard Curve.)

Typical Testing Data/Standard Curve (for reference only) (Fig.1. Human Solute carrier family 15 member 1 (SLC15A1) Standard Curve.)
Related Product Information for SLC15A1 elisa kit
Description: This Human Solute carrier family 15 member 1 (SLC15A1) ELISA Kit employs a two-site sandwich ELISA to quantitate SLC15A1 in samples. An antibody specific for SLC15A1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any SLC15A1 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for SLC15A1 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of SLC15A1 bound in the initial step. The color development is stopped and the intensity of the color is measured.

Background: SLC15A1 encodes an intestinal hydrogen peptide cotransporter that is a member of the solute carrier family 15. The encoded protein is localized to the brush border membrane of the intestinal epithelium and mediates the uptake of di- and tripeptides from the lumen into the enterocytes. This protein plays an important role in the uptake and digestion of dietary proteins. This protein also facilitates the absorption of numerous peptidomimetic drugs.Studies indicated that it transports dipeptides and tripeptides but not free amino acids or peptides with more than 3 amino acid residues and that its driving force for uphill transport requires proton binding and presence of an inside-negative membrane potential. A membrane protein, HTP1, which appeared to be associated with the oligopeptide transporter, had also been cloned

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
NCBI Official Full Name
solute carrier family 15 member 1
NCBI Official Synonym Full Names
solute carrier family 15 member 1
NCBI Official Symbol
SLC15A1
NCBI Official Synonym Symbols
PEPT1; HPECT1; HPEPT1
NCBI Protein Information
solute carrier family 15 member 1
UniProt Protein Name
Solute carrier family 15 member 1
Protein Family
UniProt Gene Name
SLC15A1
UniProt Synonym Gene Names
PEPT1
UniProt Entry Name
S15A1_HUMAN

NCBI Description

This gene encodes an intestinal hydrogen peptide cotransporter that is a member of the solute carrier family 15. The encoded protein is localized to the brush border membrane of the intestinal epithelium and mediates the uptake of di- and tripeptides from the lumen into the enterocytes. This protein plays an important role in the uptake and digestion of dietary proteins. This protein also facilitates the absorption of numerous peptidomimetic drugs. [provided by RefSeq, Apr 2010]

Uniprot Description

SLC15A1: Proton-coupled intake of oligopeptides of 2 to 4 amino acids with a preference for dipeptides. May constitute a major route for the absorption of protein digestion end-products. Belongs to the PTR2/POT transporter (TC 2.A.17) family.

Protein type: Transporter; Membrane protein, multi-pass; Transporter, SLC family; Membrane protein, integral

Chromosomal Location of Human Ortholog: 13q32.3

Cellular Component: membrane; integral to plasma membrane; plasma membrane

Molecular Function: proton-dependent oligopeptide secondary active transmembrane transporter activity; peptide:hydrogen symporter activity

Biological Process: proton transport; protein transport; transport; digestion; ion transport; transmembrane transport

Research Articles on SLC15A1

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Product Notes

The Human SLC15A1 slc15a1 (Catalog #AAA9714293) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA9714293 ELISA Kit recognizes Human SLC15A1. It is sometimes possible for the material contained within the vial of "Solute Carrier Family 15 Member 1 (SLC15A1), ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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