CBS sirna

CBS siRNA (Mouse)

Cat. Num. AAA8224440

• Gene Names: Cbs; HIP4; AI047524; AI303044
• Reactivity: Mouse
• Applications: RNA Interference (RNAi)
• Purity: > 97%
• Synonyms: CBS; CBS siRNA (Mouse); Cystathionine beta-synthase; Beta-thionase; Serine sulfhydrase; CBS sirna

• Host: Synthetic
• Reactivity: Mouse
• Specificity: CBS siRNA (Mouse) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
• Purity/Purification: > 97%
• Form/Format: Lyophilized powder
• Sequence Length: 547

• Applicable Applications for CBS sirna: RNA Interference (RNAi)
• Quality Control: Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
• Directions for Use: We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
• Components: We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of mouse CBS gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
• Preparation and Storage: Shipped at 4 degree C. Store at -20 degree C for one year.

Related Product Information for CBS sirna

siRNA to inhibit CBS expression using RNA interference

NCBI and Uniprot Product Information

• NCBI GI #: 405778333
• NCBI GeneID: 12411
• NCBI Accession #: NP_001258282.1
• NCBI GenBank Nucleotide #: NM_001271353.1
• UniProt Accession #: Q91WT9; Q91WU3
• Molecular Weight: 60,193 Da
• NCBI Official Full Name: cystathionine beta-synthase isoform 2
• NCBI Official Synonym Full Names: cystathionine beta-synthase
• NCBI Official Symbol: Cbs
• NCBI Official Synonym Symbols: HIP4; AI047524; AI303044
• NCBI Protein Information: cystathionine beta-synthase
• UniProt Protein Name: Cystathionine beta-synthase
• Protein Family: Cystathionine beta-synthase
• UniProt Gene Name: Cbs
• UniProt Entry Name: CBS_MOUSE

Uniprot Description

CBS: Only known pyridoxal phosphate-dependent enzyme that contains heme. Important regulator of hydrogen sulfide, especially in the brain, utilizing cysteine instead of serine to catalyze the formation of hydrogen sulfide. Hydrogen sulfide is a gastratransmitter with signaling and cytoprotective effects such as acting as a neuromodulator in the brain to protect neurons against hypoxic injury. Defects in CBS are the cause of cystathionine beta- synthase deficiency (CBSD). CBSD is an enzymatic deficiency resulting in altered sulfur metabolism and homocystinuria. The clinical features of untreated homocystinuria due to CBS deficiency include myopia, ectopia lentis, mental retardation, skeletal anomalies resembling Marfan syndrome, and thromboembolic events. Light skin and hair can also be present. Biochemical features include increased urinary homocystine and methionine. Belongs to the cysteine synthase/cystathionine beta- synthase family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Lyase; Amino Acid Metabolism - glycine, serine and threonine; EC 4.2.1.22; Other Amino Acids Metabolism - selenoamino acid; Amino Acid Metabolism - cysteine and methionine

Cellular Component: intracellular membrane-bound organelle; cytoplasm; nucleolus; cytosol; nucleus

Molecular Function: identical protein binding; enzyme binding; protein homodimerization activity; cystathionine beta-synthase activity; metal ion binding; ubiquitin protein ligase binding; heme binding; lyase activity; pyridoxal phosphate binding

Biological Process: maternal process involved in pregnancy; transsulfuration; cysteine biosynthetic process; cysteine biosynthetic process from serine; superoxide metabolic process; cerebellum morphogenesis; homocysteine metabolic process; cysteine biosynthetic process via cystathionine; response to folic acid; regulation of JNK activity; blood vessel remodeling; regulation of cGMP metabolic process; amino acid biosynthetic process; regulation of blood vessel size; negative regulation of apoptosis; endochondral ossification