C8A sirna

C8A siRNA (Mouse)

Cat. Num. AAA8236145

• Reactivity: Mouse
• Applications: RNA Interference (RNAi)
• Purity: > 97%
• Synonyms: C8A; C8A siRNA (Mouse); Complement component C8 alpha chain; Complement component 8 subunit alpha; C8A sirna

• Host: Synthetic
• Reactivity: Mouse
• Specificity: C8A siRNA (Mouse) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
• Purity/Purification: > 97%
• Form/Format: Lyophilized powder
• Sequence Length: 587

• Applicable Applications for C8A sirna: RNA Interference (RNAi)
• Quality Control: Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
• Directions for Use: We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
• Components: We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of mouse C8A gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
• Preparation and Storage: Shipped at 4 degree C. Store at -20 degree C for one year.

Related Product Information for C8A sirna

siRNA to inhibit C8A expression using RNA interference

NCBI and Uniprot Product Information

• NCBI GI #: 22122667
• NCBI GeneID: 230558
• NCBI Accession #: NP_666260.1
• NCBI GenBank Nucleotide #: NM_146148.2
• UniProt Accession #: Q8K182; Q8CHJ9; Q8CHK1
• Molecular Weight: 66,080 Da
• NCBI Official Full Name: complement component C8 alpha chain isoform 1
• NCBI Official Synonym Full Names: complement component 8, alpha polypeptide
• NCBI Official Symbol: C8a
• NCBI Protein Information: complement component C8 alpha chain
• UniProt Protein Name: Complement component C8 alpha chain
• Protein Family: Complement component
• UniProt Gene Name: C8a
• UniProt Entry Name: CO8A_MOUSE

Uniprot Description

C8A: Constituent of the membrane attack complex (MAC) that plays a key role in the innate and adaptive immune response by forming pores in the plasma membrane of target cells. C8A inserts into the target membrane, but does not form pores by itself. Defects in C8A are a cause of complement component 8 deficiency type 1 (C8D1). A rare defect of the complement classical pathway associated with susceptibility to severe recurrent infections, predominantly by Neisseria gonorrhoeae or Neisseria meningitidis. Belongs to the complement C6/C7/C8/C9 family.

Protein type: Membrane protein, multi-pass

Cellular Component: membrane attack complex; extracellular space; membrane; plasma membrane; extracellular region; integral to membrane

Molecular Function: complement binding; protein complex binding

Biological Process: immune system process; cytolysis; complement activation, alternative pathway; innate immune response; immune response; complement activation, classical pathway; complement activation