BRAF sirna

BRAF siRNA (Mouse)

Cat. Num. AAA8236822

• Gene Names: Braf; B-raf; Braf2; Braf-2; C87398; AA120551; AA387315; AA473386; C230098H17; D6Ertd631e; 9930012E13Rik
• Reactivity: Mouse
• Applications: RNA Interference (RNAi)
• Purity: > 97%
• Synonyms: BRAF; BRAF siRNA (Mouse); B-RAF; Serine/threonine-protein kinase B-raf; Proto-oncogene B-Raf; BRAF sirna

• Host: Synthetic
• Reactivity: Mouse
• Specificity: BRAF siRNA (Mouse) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
• Purity/Purification: > 97%
• Form/Format: Lyophilized powder
• Sequence Length: 329

• Applicable Applications for BRAF sirna: RNA Interference (RNAi)
• Quality Control: Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
• Directions for Use: We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
• Components: We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of mouse BRAF gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
• Preparation and Storage: Shipped at 4 degree C. Store at -20 degree C for one year.

Related Product Information for BRAF sirna

siRNA to inhibit BRAF expression using RNA interference

NCBI and Uniprot Product Information

• NCBI GI #: 153791904
• NCBI GeneID: 109880
• NCBI Accession #: NP_647455.3
• NCBI GenBank Nucleotide #: NM_139294.5
• UniProt Accession #: P28028; Q3USE9; E9QNG9
• Molecular Weight: 37,118 Da
• NCBI Official Full Name: serine/threonine-protein kinase B-raf
• NCBI Official Synonym Full Names: Braf transforming gene
• NCBI Official Symbol: Braf
• NCBI Official Synonym Symbols: B-raf; Braf2; Braf-2; C87398; AA120551; AA387315; AA473386; C230098H17; D6Ertd631e; 9930012E13Rik
• NCBI Protein Information: serine/threonine-protein kinase B-raf
• UniProt Protein Name: Serine/threonine-protein kinase B-raf
• Protein Family: Serine/threonine-protein kinase
• UniProt Gene Name: Braf
• UniProt Synonym Gene Names: B-raf
• UniProt Entry Name: BRAF_MOUSE

Uniprot Description

BRAF: a tyrosine kinase-like kinase of the RAF family. Involved in the transduction of mitogenic signals from the cell membrane to the nucleus. May play a role in the postsynaptic responses of hippocampal neuron. Frequently mutated in thyroid cancers, skin melanomas and at lower frequency in a wide range of human cancers. An activating mutation, mimicking phosphorylation of the activation loop, is seen in 60% of malignant melanoma samples. Raf mutations are generally exclusive to Ras activating mutations. Activating mutations are also seen in ~10% of colorectal cancers, in lung cancers and gliomas, and at a lower rate in several other tumors. Inactivating mutations are also seen and may result in activation of c-Raf and Erk. Mutations in B-Raf, MEK1 and MEK2 also associated with cardiofaciocutaneous syndrome, displaying morphological, cardiac and mental defects. Approved Inhibitor: Nexavar/Sorafenib.

Protein type: Oncoprotein; Protein kinase, Ser/Thr (non-receptor); Kinase, protein; EC 2.7.11.1; Protein kinase, TKL; TKL group; RAF family

Cellular Component: neuron projection; membrane; mitochondrion; cytoplasm; plasma membrane; nucleus

Molecular Function: identical protein binding; mitogen-activated protein kinase kinase binding; metal ion binding; nucleotide binding; calcium ion binding; protein kinase activity; transferase activity; protein serine/threonine kinase activity; protein binding; MAP kinase kinase kinase activity; protein heterodimerization activity; transferase activity, transferring phosphorus-containing groups; receptor signaling protein activity; kinase activity; ATP binding

Biological Process: positive T cell selection; response to peptide hormone stimulus; positive regulation of axon regeneration; response to cAMP; activation of MAPKK activity; CD4-positive, alpha beta T cell differentiation; somatic stem cell maintenance; protein heterooligomerization; MAPKKK cascade; alpha-beta T cell differentiation; signal transduction; positive regulation of peptidyl-serine phosphorylation; protein amino acid phosphorylation; regulation of cell proliferation; regulation of axon regeneration; myeloid progenitor cell differentiation; positive regulation of stress fiber formation; visual learning; positive regulation of axonogenesis; negative regulation of neuron apoptosis; cell differentiation; phosphorylation; negative regulation of apoptosis