BMP10 sirna

BMP10 siRNA (Mouse)

Cat. Num. AAA8207226

• Reactivity: Mouse
• Applications: RNA Interference (RNAi)
• Purity: > 97%
• Synonyms: BMP10; BMP10 siRNA (Mouse); Bone morphogenetic protein 10; BMP-10; BMP10 sirna

• Host: Synthetic
• Reactivity: Mouse
• Specificity: BMP10 siRNA (Mouse) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
• Purity/Purification: > 97%
• Form/Format: Lyophilized powder
• Sequence Length: 421

• Applicable Applications for BMP10 sirna: RNA Interference (RNAi)
• Quality Control: Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
• Directions for Use: We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
• Components: We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of mouse BMP10 gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
• Preparation and Storage: Shipped at 4 degree C. Store at -20 degree C for one year.

Related Product Information for BMP10 sirna

siRNA to inhibit BMP10 expression using RNA interference

NCBI and Uniprot Product Information

• NCBI GI #: 157837977
• NCBI GeneID: 12154
• NCBI Accession #: NP_033886.2
• NCBI GenBank Nucleotide #: NM_009756.3
• UniProt Accession #: Q9R229; Q9Z1V8; A6H6S5
• Molecular Weight: 47,888 Da
• NCBI Official Full Name: bone morphogenetic protein 10 preproprotein
• NCBI Official Synonym Full Names: bone morphogenetic protein 10
• NCBI Official Symbol: Bmp10
• NCBI Protein Information: bone morphogenetic protein 10
• UniProt Protein Name: Bone morphogenetic protein 10
• Protein Family: Bone morphogenetic protein
• UniProt Gene Name: Bmp10
• UniProt Synonym Gene Names: BMP-10
• UniProt Entry Name: BMP10_MOUSE

NCBI Description

This gene encodes a member of the TGF-beta family of growth factors and preproprotein that is proteolytically processed to generate a mature protein product. This protein product binds to the activin receptor-like kinase 1 (ALK1) and reportedly plays a role in diverse processes in cardiovascular development including cardiomyocyte proliferation, closure of the ductus arteriosus and angiogenesis. Homozygous knockout mice for this gene exhibit impaired heart development and embryonic lethality. [provided by RefSeq, Aug 2015]

Uniprot Description

BMP10: Required for maintaining the proliferative activity of embryonic cardiomyocytes by preventing premature activation of the negative cell cycle regulator CDKN1C/p57KIP and maintaining the required expression levels of cardiogenic factors such as MEF2C and NKX2-5. Acts as a ligand for ACVRL1/ALK1, BMPR1A/ALK3 and BMPR1B/ALK6, leading to activation of SMAD1, SMAD5 and SMAD8 transcription factors. Inhibits endothelial cell migration and growth. Belongs to the TGF-beta family.

Protein type: Secreted, signal peptide; Secreted

Cellular Component: extracellular space; cell surface; cytoplasm; extracellular region; Z disc

Molecular Function: receptor serine/threonine kinase binding; growth factor activity; telethonin binding; hormone activity; cytokine activity; transforming growth factor beta receptor binding

Biological Process: adult heart development; heart development; multicellular organismal development; positive regulation of transcription, DNA-dependent; ventricular cardiac muscle cell development; activin receptor signaling pathway; cardiac muscle cell proliferation; sarcomere organization; BMP signaling pathway; regulation of apoptosis; regulation of MAPKKK cascade; ventricular cardiac muscle morphogenesis; negative regulation of cell growth; cell adhesion; negative regulation of cell migration; positive regulation of cardiac muscle cell proliferation; growth