BMP1 sirna

BMP1 siRNA (Mouse)

Cat. Num. AAA8229277

• Gene Names: Bmp1; Pcp; Tld
• Reactivity: Mouse
• Applications: RNA Interference (RNAi)
• Purity: > 97%
• Synonyms: BMP1; BMP1 siRNA (Mouse); Bone morphogenetic protein 1; BMP-1; Mammalian tolloid protein; mTld; Procollagen C-proteinase; PCP; BMP1 sirna

• Host: Synthetic
• Reactivity: Mouse
• Specificity: BMP1 siRNA (Mouse) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
• Purity/Purification: > 97%
• Form/Format: Lyophilized powder
• Sequence Length: 991

• Applicable Applications for BMP1 sirna: RNA Interference (RNAi)
• Quality Control: Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
• Directions for Use: We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
• Components: We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of mouse BMP1 gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
• Preparation and Storage: Shipped at 4 degree C. Store at -20 degree C for one year.

Related Product Information for BMP1 sirna

siRNA to inhibit BMP1 expression using RNA interference

NCBI and Uniprot Product Information

• NCBI GI #: 42734447
• NCBI GeneID: 12153
• NCBI Accession #: NP_033885.2
• NCBI GenBank Nucleotide #: NM_009755.3
• UniProt Accession #: P98063; Q6NZM2
• Molecular Weight: 111,666 Da
• NCBI Official Full Name: bone morphogenetic protein 1
• NCBI Official Synonym Full Names: bone morphogenetic protein 1
• NCBI Official Symbol: Bmp1
• NCBI Official Synonym Symbols: Pcp; Tld
• NCBI Protein Information: bone morphogenetic protein 1
• UniProt Protein Name: Bone morphogenetic protein 1
• Protein Family: Bone morphogenetic protein
• UniProt Gene Name: Bmp1
• UniProt Synonym Gene Names: BMP-1; mTld; PCP
• UniProt Entry Name: BMP1_MOUSE

NCBI Description

This gene encodes a metalloproteinase that plays an essential role in the formation of the extracellular matrix and is also able to induce ectopic bone formation. Unlike other bone morphogenetic proteins, the protein encoded by this gene is not closely related to transforming growth factor-beta. This protein plays in role several developmental processes. In humans, mutations in this gene are associated with osteogenesis imperfecta and with increased bone mineral density and multiple recurrent fractures. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Dec 2013]

Uniprot Description

BMP1: Cleaves the C-terminal propeptides of procollagen I, II and III. Induces cartilage and bone formation. May participate in dorsoventral patterning during early development by cleaving chordin (CHRD). Defects in BMP1 are a cause of autosomal recessive osteogenesis imperfecta (AR-OI). A connective tissue disorder characterized by bone fragility, progressively deforming bones, bowing of limbs due to multiple fractures, very short stature, a triangular face, severe scoliosis, and grayish sclera. AR-OI due to BMP1 mutations belongs to the group of osteogenesis imperfecta type III in the Sillence classification. Belongs to the peptidase M12A family. 7 isoforms of the human protein are produced by alternative splicing.

Protein type: Cytokine; Protease; EC 3.4.24.19

Cellular Component: Golgi apparatus; extracellular space; proteinaceous extracellular matrix; extracellular region

Molecular Function: peptidase activity; protein binding; growth factor activity; metallopeptidase activity; hydrolase activity; zinc ion binding; metal ion binding; metalloendopeptidase activity; cytokine activity; calcium ion binding

Biological Process: ossification; multicellular organismal development; cartilage development; cell differentiation; proteolysis