BLNK sirna

BLNK siRNA (Mouse)

Cat. Num. AAA8239572

• Gene Names: Blnk; Bca; BASH; Ly57; Ly-57; Lyw-57; SLP-65
• Reactivity: Mouse
• Applications: RNA Interference (RNAi)
• Purity: > 97%
• Synonyms: BLNK; BLNK siRNA (Mouse); BASH; LY57; SLP65; B-cell linker protein; B-cell adapter containing a SH2 domain protein; B-cell adapter containing a Src homology 2 domain protein; Cytoplasmic adapter protein; Lymphocyte antigen 57; Src homology 2 domain-containing leukocyte protein of 65 kDa; Slp-65; BLNK sirna

• Host: Synthetic
• Reactivity: Mouse
• Specificity: BLNK siRNA (Mouse) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
• Purity/Purification: > 97%
• Form/Format: Lyophilized powder
• Sequence Length: 457

• Applicable Applications for BLNK sirna: RNA Interference (RNAi)
• Quality Control: Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
• Directions for Use: We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
• Components: We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of mouse BLNK gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
• Preparation and Storage: Shipped at 4 degree C. Store at -20 degree C for one year.

Related Product Information for BLNK sirna

siRNA to inhibit BLNK expression using RNA interference

NCBI and Uniprot Product Information

• NCBI GI #: 31982209
• NCBI GeneID: 17060
• NCBI Accession #: NP_032554.2
• NCBI GenBank Nucleotide #: NM_008528.4
• UniProt Accession #: Q9QUN3; O88504
• Molecular Weight: 50,671 Da
• NCBI Official Full Name: B-cell linker protein
• NCBI Official Synonym Full Names: B cell linker
• NCBI Official Symbol: Blnk
• NCBI Official Synonym Symbols: Bca; BASH; Ly57; Ly-57; Lyw-57; SLP-65
• NCBI Protein Information: B-cell linker protein
• UniProt Protein Name: B-cell linker protein
• Protein Family: B-cell linker protein
• UniProt Gene Name: Blnk
• UniProt Synonym Gene Names: Bash; Ly57; Slp65; Slp-65
• UniProt Entry Name: BLNK_MOUSE

Uniprot Description

BLNK: an adaptor protein that bridges the B-cell receptor-associated kinases (BCR) with a multitude of signaling pathways, regulating biologic outcomes of B-cell function and development. Plays an important role in BCR-mediated PLCG1 activation and Ca(2 ) mobilization. Does not seem to be not required for pre-BCR-mediated activation of MAP kinase and phosphatidyl-inositol 3 (PI3) kinase signaling. Plays a critical role in orchestrating the pro-B cell to pre-B cell transition. Following BCR activation, phosphorylated on tyrosine residues by SYK and LYN. When phosphorylated, serves as a scaffold to assemble downstream targets of antigen activation, including PLCG1, VAV1, GRB2 and NCK1. Its phosphorylation is required for both Ca(2 ) and MAPK signaling pathways. Defects in BLNK are the cause of hypoglobulinemia and absent B-cells.It has tumor supressor activity that is lost in many cases of childhood pre-B acute lymphoblastic leukemia (ALL). Two alternatively spliced isoforms have been described; these are differentially involved in activation and apoptosis of B lymphocytes.

Protein type: Adaptor/scaffold

Cellular Component: membrane; cytoplasm; plasma membrane; intracellular

Molecular Function: protein binding; SH3/SH2 adaptor activity

Biological Process: positive regulation of signal transduction; B cell activation