BIRC5 sirna

BIRC5 siRNA (Mouse)

Cat. Num. AAA8211741

• Gene Names: Birc5; Api4; TIAP; AAC-11; survivin40
• Reactivity: Mouse
• Applications: RNA Interference (RNAi)
• Purity: > 97%
• Synonyms: BIRC5; BIRC5 siRNA (Mouse); API4; IAP4; Baculoviral IAP repeat-containing protein 5; Apoptosis inhibitor 4; Apoptosis inhibitor survivin; TIAP; BIRC5 sirna

• Host: Synthetic
• Reactivity: Mouse
• Specificity: BIRC5 siRNA (Mouse) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
• Purity/Purification: > 97%
• Form/Format: Lyophilized powder
• Sequence Length: 121

• Applicable Applications for BIRC5 sirna: RNA Interference (RNAi)
• Quality Control: Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
• Directions for Use: We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
• Components: We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of mouse BIRC5 gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
• Preparation and Storage: Shipped at 4 degree C. Store at -20 degree C for one year.

Related Product Information for BIRC5 sirna

siRNA to inhibit BIRC5 expression using RNA interference

NCBI and Uniprot Product Information

• NCBI GI #: 59859890
• NCBI GeneID: 11799
• NCBI Accession #: NP_001012273.1
• NCBI GenBank Nucleotide #: NM_001012273.1
• UniProt Accession #: O70201; Q923F7; Q9WU53; Q9WU54
• Molecular Weight: 4,670 Da
• NCBI Official Full Name: baculoviral IAP repeat-containing protein 5 isoform 3
• NCBI Official Synonym Full Names: baculoviral IAP repeat-containing 5
• NCBI Official Symbol: Birc5
• NCBI Official Synonym Symbols: Api4; TIAP; AAC-11; survivin40
• NCBI Protein Information: baculoviral IAP repeat-containing protein 5
• UniProt Protein Name: Baculoviral IAP repeat-containing protein 5
• Protein Family: Baculoviral IAP repeat-containing protein
• UniProt Gene Name: Birc5
• UniProt Synonym Gene Names: Api4; Iap4
• UniProt Entry Name: BIRC5_MOUSE

NCBI Description

This gene is a member of the inhibitor of apoptosis (IAP) gene family, which encode negative regulatory proteins that prevent apoptotic cell death. IAP family members usually contain multiple baculovirus IAP repeat (BIR) domains, but this gene encodes proteins with only a single BIR domain. The encoded proteins also lack a C-terminus RING finger domain. In humans, gene expression is high during fetal development and in most tumors yet low in adult tissues. Antisense transcripts have been identified in human that regulate this gene's expression. At least three transcript variants encoding distinct isoforms have been found for this gene, although at least one of these transcript variants is a nonsense-mediated decay (NMD) candidate. [provided by RefSeq, Jul 2008]

Uniprot Description

Survivin: an apoptosis inhibitor that is expressed during the G2/M phase of the cell cycle. Associates with the microtubules of the mitotic spindle and any disruption results in the loss of apoptosis activity. May play a role in neoplasia. Inhibitor of caspase-3 and caspase-7. Two splice variant isoforms have been described.

Protein type: Apoptosis

Cellular Component: nuclear chromosome; microtubule; chromosome; interphase microtubule organizing center; cytosol; kinetochore; centriole; cytoskeleton; cytoplasmic microtubule; spindle microtubule; cytoplasm; apical plasma membrane; midbody; nucleus; chromosome, pericentric region

Molecular Function: tubulin binding; identical protein binding; protein homodimerization activity; zinc ion binding; protease inhibitor activity; microtubule binding; metal ion binding; caspase inhibitor activity; ubiquitin-protein ligase activity; protein binding; enzyme binding; protein heterodimerization activity; chaperone binding; cofactor binding; cysteine protease inhibitor activity; Ran GTPase binding

Biological Process: apoptosis; regulation of mitotic cell cycle; protein ubiquitination; regulation of signal transduction; cytokinesis; negative regulation of peptidase activity; negative regulation of caspase activity; positive regulation of mitotic cell cycle; protein amino acid phosphorylation; chromosome segregation; regulation of transcription, DNA-dependent; negative regulation of neuron apoptosis; G2/M transition of mitotic cell cycle; positive regulation of exit from mitosis; mitosis; spindle checkpoint; transcription, DNA-dependent; establishment of chromosome localization; positive regulation of cell cycle; microtubule cytoskeleton organization and biogenesis; cell cycle; embryonic development; protein complex localization; cell division; negative regulation of transcription, DNA-dependent; negative regulation of apoptosis