BBS9 sirna

BBS9 siRNA (Mouse)

Cat. Num. AAA8207548

• Gene Names: Bbs9; E130103I17Rik
• Reactivity: Mouse
• Applications: RNA Interference (RNAi)
• Purity: > 97%
• Synonyms: BBS9; BBS9 siRNA (Mouse); PTHB1; Protein PTHB1; Bardet-Biedl syndrome 9 protein homolog; Parathyroid hormone-responsive B1 gene protein homolog; BBS9 sirna

• Host: Synthetic
• Reactivity: Mouse
• Specificity: BBS9 siRNA (Mouse) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
• Purity/Purification: > 97%
• Form/Format: Lyophilized powder
• Sequence Length: 309

• Applicable Applications for BBS9 sirna: RNA Interference (RNAi)
• Quality Control: Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
• Directions for Use: We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
• Components: We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of mouse BBS9 gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
• Preparation and Storage: Shipped at 4 degree C. Store at -20 degree C for one year.

Related Product Information for BBS9 sirna

siRNA to inhibit BBS9 expression using RNA interference

NCBI and Uniprot Product Information

• NCBI GI #: 189339274
• NCBI GeneID: 319845
• NCBI Accession #: NP_848502.1
• NCBI GenBank Nucleotide #: NM_178415.1
• UniProt Accession #: Q811G0; P97498; Q3UMR2; Q7TQH9; Q8BKD7; Q8K0T5
• Molecular Weight: 34,996 Da
• NCBI Official Full Name: protein PTHB1
• NCBI Official Synonym Full Names: Bardet-Biedl syndrome 9 (human)
• NCBI Official Symbol: Bbs9
• NCBI Official Synonym Symbols: E130103I17Rik
• NCBI Protein Information: protein PTHB1
• UniProt Protein Name: Protein PTHB1
• Protein Family: Protein
• UniProt Gene Name: Bbs9
• UniProt Synonym Gene Names: Pthb1
• UniProt Entry Name: PTHB1_MOUSE

Uniprot Description

BBS9: The BBSome complex is required for ciliogenesis but is dispensable for centriolar satellite function. This ciliogenic function is mediated in part by the Rab8 GDP/GTP exchange factor, which localizes to the basal body and contacts the BBSome. Rab8(GTP) enters the primary cilium and promotes extension of the ciliary membrane. Firstly the BBSome associates with the ciliary membrane and binds to RAB3IP/Rabin8, the guanosyl exchange factor (GEF) for Rab8 and then the Rab8-GTP localizes to the cilium and promotes docking and fusion of carrier vesicles to the base of the ciliary membrane. A chromosomal aberration involving PTHB1 is found in Wilms tumor 5 (WT5). Translocation t(1;7)(q42;p15) with OBSCN. Defects in BBS9 are a cause of Bardet-Biedl syndrome type 9 (BBS9). Bardet-Biedl syndrome (BBS) is a genetically heterogeneous, autosomal recessive disorder characterized by usually severe pigmentary retinopathy, early onset obesity, polydactyly, hypogenitalism, renal malformation and mental retardation. 6 isoforms of the human protein are produced by alternative splicing.

Cellular Component: pericentriolar material; cytoskeleton; cell projection; membrane; cytoplasm; plasma membrane; cilium

Molecular Function: protein binding

Biological Process: protein transport; cell projection organization and biogenesis; transport; cilium biogenesis