BAIAP2 sirna

BAIAP2 siRNA (Mouse)

Cat. Num. AAA8208056

• Gene Names: Baiap2; IRSp53; R75030
• Reactivity: Mouse
• Applications: RNA Interference (RNAi)
• Purity: > 97%
• Synonyms: BAIAP2; BAIAP2 siRNA (Mouse); Brain-specific angiogenesis inhibitor 1-associated protein 2; BAI-associated protein 2; BAI1-associated protein 2; Insulin receptor substrate protein of 53 kDa; IRSp53; Insulin receptor substrate p53; Insulin receptor tyrosine kinase 53 kDa substrate; BAIAP2 sirna

• Host: Synthetic
• Reactivity: Mouse
• Specificity: BAIAP2 siRNA (Mouse) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
• Purity/Purification: > 97%
• Form/Format: Lyophilized powder
• Sequence Length: 482

• Applicable Applications for BAIAP2 sirna: RNA Interference (RNAi)
• Quality Control: Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
• Directions for Use: We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
• Components: We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of mouse BAIAP2 gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
• Preparation and Storage: Shipped at 4 degree C. Store at -20 degree C for one year.

Related Product Information for BAIAP2 sirna

siRNA to inhibit BAIAP2 expression using RNA interference

NCBI and Uniprot Product Information

• NCBI GI #: 83649713
• NCBI GeneID: 108100
• NCBI Accession #: NP_001032843.1
• NCBI GenBank Nucleotide #: NM_001037754.3
• UniProt Accession #: Q8BKX1; Q91V97; Q923V9; Q923W0
• Molecular Weight: 53,274 Da
• NCBI Official Full Name: brain-specific angiogenesis inhibitor 1-associated protein 2 isoform c
• NCBI Official Synonym Full Names: brain-specific angiogenesis inhibitor 1-associated protein 2
• NCBI Official Symbol: Baiap2
• NCBI Official Synonym Symbols: IRSp53; R75030
• NCBI Protein Information: brain-specific angiogenesis inhibitor 1-associated protein 2
• UniProt Protein Name: Brain-specific angiogenesis inhibitor 1-associated protein 2
• Protein Family: Brain-specific angiogenesis inhibitor 1-associated protein
• UniProt Gene Name: Baiap2
• UniProt Synonym Gene Names: BAI-associated protein 2; BAI1-associated protein 2; IRSp53; Insulin receptor substrate p53
• UniProt Entry Name: BAIP2_MOUSE

Uniprot Description

IRSp53: Adapter protein that links membrane-bound small G- proteins to cytoplasmic effector proteins. Necessary for CDC42- mediated reorganization of the actin cytoskeleton and for RAC1- mediated membrane ruffling. Involved in the regulation of the actin cytoskeleton by WASF family members and the Arp2/3 complex. Plays a role in neurite growth. Acts syngeristically with ENAH to promote filipodia formation. Plays a role in the reorganization of the actin cytoskeleton in response to bacterial infection. Homodimer. Interacts with CDC42 and RAC1 that have been activated by GTP binding. Interacts with ATN1, BAI1, EPS8, SHANK1, SHANK2, SHANK3, WASF1 and WASF2. Interacts with ENAH after recruitment of CDC42. Interacts with TIAM1 and DIAPH1. Interacts (via SH3 domain) with E.coli effector protein EspF(U) (via PXXP motifs). Interacts with E.coli intimin receptor Tir. Isoform 1 and isoform 4 are expressed almost exclusively in brain. Isoform 4 is barely detectable in placenta, prostate and testis. A short isoform is ubiquitous, with the highest expression in liver, prostate, testis and placenta. 6 isoforms of the human protein are produced by alternative splicing.

Protein type: Actin-binding; Motility/polarity/chemotaxis

Cellular Component: neuron projection; postsynaptic density; dendritic spine; cytosol; actin cytoskeleton; secretory granule; nucleoplasm; cell projection; cytoskeleton; cell soma; membrane; cytoplasm; plasma membrane; intracellular

Molecular Function: identical protein binding; protein binding; cytoskeletal adaptor activity; PDZ domain binding

Biological Process: actin crosslink formation; regulation of cell shape; actin filament bundle formation; response to bacterium; plasma membrane organization and biogenesis; regulation of actin cytoskeleton organization and biogenesis; dendrite development; regulation of synaptic plasticity; Rho protein signal transduction