ATP6V0A4 sirna

ATP6V0A4 siRNA (Mouse)

Cat. Num. AAA8216449

• Gene Names: Atp6v0a4; a4; Atp6n1b
• Reactivity: Mouse
• Applications: RNA Interference (RNAi)
• Purity: > 97%
• Synonyms: ATP6V0A4; ATP6V0A4 siRNA (Mouse); ATP6N1B; V-type proton ATPase 116 kDa subunit a isoform 4; V-ATPase 116 kDa isoform a4; Vacuolar proton translocating ATPase 116 kDa subunit a isoform 4; Vacuolar proton translocating ATPase 116 kDa subunit a kidney isoform; ATP6V0A4 sirna

• Host: Synthetic
• Reactivity: Mouse
• Specificity: ATP6V0A4 siRNA (Mouse) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
• Purity/Purification: > 97%
• Form/Format: Lyophilized powder
• Sequence Length: 833

• Applicable Applications for ATP6V0A4 sirna: RNA Interference (RNAi)
• Quality Control: Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
• Directions for Use: We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
• Components: We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of mouse ATP6V0A4 gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
• Preparation and Storage: Shipped at 4 degree C. Store at -20 degree C for one year.

Related Product Information for ATP6V0A4 sirna

siRNA to inhibit ATP6V0A4 expression using RNA interference

NCBI and Uniprot Product Information

• NCBI GI #: 171543866
• NCBI GeneID: 140494
• NCBI Accession #: NP_536715.3
• NCBI GenBank Nucleotide #: NM_080467.3
• UniProt Accession #: Q920R6; Q8CJ79; Q920B5
• Molecular Weight: 95,605 Da
• NCBI Official Full Name: V-type proton ATPase 116 kDa subunit a isoform 4
• NCBI Official Synonym Full Names: ATPase, H+ transporting, lysosomal V0 subunit A4
• NCBI Official Symbol: Atp6v0a4
• NCBI Official Synonym Symbols: a4; Atp6n1b
• NCBI Protein Information: V-type proton ATPase 116 kDa subunit a isoform 4
• UniProt Protein Name: V-type proton ATPase 116 kDa subunit a isoform 4
• Protein Family: V-type proton ATPase
• UniProt Gene Name: Atp6v0a4
• UniProt Synonym Gene Names: Atp6n1b; V-ATPase 116 kDa isoform a4
• UniProt Entry Name: VPP4_MOUSE

Uniprot Description

ATP6V0A4: Part of the proton channel of the V-ATPase that is involved in normal vectorial acid transport into the urine by the kidney. Defects in ATP6V0A4 are the cause of distal renal tubular acidosis with preserved hearing (RTADR). RTADR is an autosomal recessive form of distal renal tubular acidosis (dRTA), a group of disorders characterized by functional failure of alpha- intercalated cells of the cortical collecting duct of the distal nephron, where vectorial proton transport is required for urinary acidification. Functional failure of alpha-intercalated cells results in metabolic acidosis accompanied by disturbances of potassium balance, urinary calcium solubility, bone physiology and growth. Belongs to the V-ATPase 116 kDa subunit family.

Protein type: Transporter; Membrane protein, integral; Energy Metabolism - oxidative phosphorylation; Transporter, ion channel; Transporter, iron; Membrane protein, multi-pass

Cellular Component: lysosomal membrane; cell; brush border membrane; integral to membrane; vacuolar proton-transporting V-type ATPase complex; membrane; apical part of cell; apical plasma membrane; plasma membrane; vacuole; intracellular; endosome; brush border

Molecular Function: hydrogen-exporting ATPase activity, phosphorylative mechanism; hydrogen ion transmembrane transporter activity; hydrogen ion transporting ATPase activity, rotational mechanism; ATPase binding

Biological Process: vacuolar acidification; ossification; ATP synthesis coupled proton transport; proton transport; sensory perception of sound; transport; ATP hydrolysis coupled proton transport; regulation of pH; ion transport; excretion