Rabbit MRPL22 Polyclonal Antibody | anti-MRPL22 antibody

MRPL22 Polyclonal Antibody

Cat. Num. AAA3007429

• Gene Names: MRPL22; L22mt; RPML25; HSPC158; MRP-L22; MRP-L25
• Reactivity: Human, Mouse, Rat
• Applications: Western Blot
• Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is >96% (by SDS-PAGE).
• Synonyms: MRPL22; Polyclonal Antibody; MRPL22 Polyclonal Antibody; 39S ribosomal protein L22; mitochondrial; L22mt; MRP-L22; 39S ribosomal protein L25; L25mt; MRP-L25; anti-MRPL22 antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: MRPL22 polyclonal antibody detects endogenous levels of MRPL22 protein.
• Purity/Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is >96% (by SDS-PAGE).
• Form/Format: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
• Sequence Length: 126

• Applicable Applications for anti-MRPL22 antibody: Western Blot (WB)
• Application Notes: WB: 1:500-1:1000
• Immunogen: Synthetic peptide, corresponding to amino acids 94-138 of Human MRPL22.
• Preparation and Storage: Store at 4 degree C short term.; Aliquot and store at -20 degree C long term.; Avoid freeze-thaw cycles.

Western Blot (WB)

(Western Blot (WB) analysis of MRPL22 polyclonal antibody at 1:500 dilutionLane1:THP-1 whole cell lysate (40ug) Lane2:Hela whole cell lysate (40ug) Lane3:HEK293T whole cell lysate (40ug) Lane4:PC12 whole cell lysate (40ug) Lane5:AML-12 whole cell lysate (40ug))

Related Product Information for anti-MRPL22 antibody

Mammalian mitochondrial ribosomes (mitoribosomes) are responsible for protein synthesis within the mitochondrion. The mitoribosomes are composed of a 4: 1 ratio of protein to RNA, with the proteins forming two subunits, the 28S subunit and the 39S subunit. Across species, the proteins that make up the mitoribosome subunits vary greatly in sequence, preventing easy recognition by sequence homology. MRP-L22 (mitochondrial ribosomal protein L22), also known as L22mt, RPML25, HSPC158 or MRP-L25, is a 206 amino acid protein that localizes to the mitochondrion, where it exists as a component of the 39S ribosomal subunit and works in conjunction with other MRPs to mediate protein synthesis. Belonging to the ribosomal protein L22P family, MRP-L22 is encoded by a gene located on human chromosome 5, which contains 181 million base pairs and comprises nearly 6% of the human genome.

NCBI and Uniprot Product Information

• NCBI GI #: 62530388
• NCBI GeneID: 29093
• NCBI Accession #: NP_001014990.1
• NCBI GenBank Nucleotide #: NP_001014990.1
• UniProt Accession #: Q9NWU5
• Molecular Weight: ~ 23kDa
• NCBI Official Full Name: 39S ribosomal protein L22, mitochondrial isoform b
• NCBI Official Synonym Full Names: mitochondrial ribosomal protein L22
• NCBI Official Symbol: MRPL22
• NCBI Official Synonym Symbols: L22mt; RPML25; HSPC158; MRP-L22; MRP-L25
• NCBI Protein Information: 39S ribosomal protein L22, mitochondrial
• UniProt Protein Name: 39S ribosomal protein L22, mitochondrial
• Protein Family: 39S ribosomal protein
• UniProt Gene Name: MRPL22
• UniProt Synonym Gene Names: MRPL25; RPML25; L22mt; MRP-L22; L25mt; MRP-L25
• UniProt Entry Name: RM22_HUMAN

NCBI Description

Mammalian mitochondrial ribosomal proteins are encoded by nuclear genes and help in protein synthesis within the mitochondrion. Mitochondrial ribosomes (mitoribosomes) consist of a small 28S subunit and a large 39S subunit. They have an estimated 75% protein to rRNA composition compared to prokaryotic ribosomes, where this ratio is reversed. Another difference between mammalian mitoribosomes and prokaryotic ribosomes is that the latter contain a 5S rRNA. Among different species, the proteins comprising the mitoribosome differ greatly in sequence, and sometimes in biochemical properties, which prevents easy recognition by sequence homology. This gene encodes a 39S subunit protein that belongs to the L22 ribosomal protein family. A pseudogene corresponding to this gene is found on chromosome 4q. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2008]

Uniprot Description

MRPL22: a mitochondrial ribosomal protein encoded by a nuclear gene that helps in protein synthesis within the mitochondrion. Mitochondrial ribosomes (mitoribosomes) consist of a small 28S subunit and a large 39S subunit. They have an estimated 75% protein to rRNA composition compared to prokaryotic ribosomes, where this ratio is reversed. Another difference between mammalian mitoribosomes and prokaryotic ribosomes is that the latter contain a 5S rRNA. Among different species, the proteins comprising the mitoribosome differ greatly in sequence, and sometimes in biochemical properties, which prevents easy recognition by sequence homology. This gene encodes a 39S subunit protein that belongs to the L22 ribosomal protein family. A pseudogene corresponding to this gene is found on chromosome 4q. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2008]

Protein type: Translation

Chromosomal Location of Human Ortholog: 5q33.2

Cellular Component: mitochondrion; mitochondrial inner membrane; large ribosomal subunit

Molecular Function: structural constituent of ribosome

Biological Process: mitochondrial translation; organelle organization and biogenesis