ATM sirna

ATM siRNA (Mouse)

Cat. Num. AAA8220150

• Gene Names: Atm; AI256621; C030026E19Rik
• Reactivity: Mouse
• Applications: RNA Interference (RNAi)
• Purity: > 97%
• Synonyms: ATM; ATM siRNA (Mouse); Serine-protein kinase ATM; Ataxia telangiectasia mutated homolog; A-T mutated homolog; ATM sirna

• Host: Synthetic
• Reactivity: Mouse
• Specificity: ATM siRNA (Mouse) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
• Purity/Purification: > 97%
• Form/Format: Lyophilized powder
• Sequence Length: 3066

• Applicable Applications for ATM sirna: RNA Interference (RNAi)
• Quality Control: Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
• Directions for Use: We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
• Components: We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of mouse ATM gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
• Preparation and Storage: Shipped at 4 degree C. Store at -20 degree C for one year.

Related Product Information for ATM sirna

siRNA to inhibit ATM expression using RNA interference

NCBI and Uniprot Product Information

• NCBI GI #: 163838660
• NCBI GeneID: 11920
• NCBI Accession #: NP_031525.2
• NCBI GenBank Nucleotide #: NM_007499.2
• UniProt Accession #: Q62388; E9QNY7
• Molecular Weight: 349,418 Da
• NCBI Official Full Name: serine-protein kinase ATM
• NCBI Official Synonym Full Names: ataxia telangiectasia mutated
• NCBI Official Symbol: Atm
• NCBI Official Synonym Symbols: AI256621; C030026E19Rik
• NCBI Protein Information: serine-protein kinase ATM
• UniProt Protein Name: Serine-protein kinase ATM
• Protein Family: ATM interactor
• UniProt Gene Name: Atm
• UniProt Synonym Gene Names: A-T mutated homolog
• UniProt Entry Name: ATM_MOUSE

Uniprot Description

ATM: an atypical kinase of the PIKK family. Regulates cell cycle checkpoints and DNA repair . May function as a tumor suppressor. Activates checkpoint signaling upon double strand breaks (DSBs), apoptosis and genotoxic stresses such as ionizing ultraviolet A light (UVA), thereby acting as a DNA damage sensor. Involved in the activation of ABL1 and SAPK. Binds DNA ends and is part of the BRCA1- associated genome surveillance complex (BASC), which contains BRCA1, MSH2, MSH6, MLH1, ATM, BLM, PMS2 and the RAD50-MRE11-NBN protein complex. This association could be a dynamic process changing throughout the cell cycle and within subnuclear domains. DNA damage promotes association with RAD17. LOF mutations associated with ataxia telangiectasia, causing progressive loss of motor control (ataxia), dilation of superficial blood vessels (telangiectasia), cancer and immune deficiency. Approximately 30% of cases develop tumors, mostly lymphomas and leukemias, due to defects in DNA damage repair. Somatic mutations seen in leukemias and lymphomas.

Protein type: Kinase, protein; Tumor suppressor; Protein kinase, Ser/Thr (non-receptor); Protein kinase, atypical; EC 2.7.11.1; DNA repair, damage; ATYPICAL group; PIKK family

Cellular Component: chromosome, telomeric region; cytoplasm; spindle; cytoplasmic vesicle; intracellular; nucleus

Molecular Function: protein dimerization activity; 1-phosphatidylinositol-3-kinase activity; nucleotide binding; protein N-terminus binding; DNA-dependent protein kinase activity; histone serine kinase activity; protein kinase activity; transferase activity; protein serine/threonine kinase activity; protein binding; DNA binding; transferase activity, transferring phosphorus-containing groups; protein complex binding; kinase activity; phosphotransferase activity, alcohol group as acceptor; ATP binding

Biological Process: lipoprotein catabolic process; DNA damage induced protein phosphorylation; regulation of cell cycle; positive regulation of apoptosis; protein amino acid autophosphorylation; heart development; histone phosphorylation; pre-B cell allelic exclusion; determination of adult life span; negative regulation of B cell proliferation; protein amino acid phosphorylation; phosphoinositide phosphorylation; positive regulation of neuron apoptosis; mitotic cell cycle spindle assembly checkpoint; cell cycle arrest; telomere maintenance; somitogenesis; V(D)J recombination; female gamete generation; cell cycle; DNA repair; double-strand break repair via homologous recombination; peptidyl-serine phosphorylation; neuron apoptosis; DNA damage response, signal transduction resulting in induction of apoptosis; DNA damage checkpoint; response to hypoxia; positive regulation of DNA damage response, signal transduction by p53 class mediator; response to ionizing radiation; brain development; phosphorylation; response to DNA damage stimulus; oocyte development