ATG7 sirna

ATG7 siRNA (Mouse)

Cat. Num. AAA8227562

• Gene Names: Atg7; Agp7; Apg7l; Atg7l; Gm21553; 1810013K23Rik
• Reactivity: Mouse
• Applications: RNA Interference (RNAi)
• Purity: > 97%
• Synonyms: ATG7; ATG7 siRNA (Mouse); APG7L; Ubiquitin-like modifier-activating enzyme ATG7; ATG12-activating enzyme E1 ATG7; Autophagy-related protein 7; APG7-like; mAGP7; Ubiquitin-activating enzyme E1-like protein; ATG7 sirna

• Host: Synthetic
• Reactivity: Mouse
• Specificity: ATG7 siRNA (Mouse) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
• Purity/Purification: > 97%
• Form/Format: Lyophilized powder
• Sequence Length: 698

• Applicable Applications for ATG7 sirna: RNA Interference (RNAi)
• Quality Control: Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
• Directions for Use: We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
• Components: We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of mouse ATG7 gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
• Preparation and Storage: Shipped at 4 degree C. Store at -20 degree C for one year.

Related Product Information for ATG7 sirna

siRNA to inhibit ATG7 expression using RNA interference

NCBI and Uniprot Product Information

• NCBI GI #: 358679369
• NCBI GeneID: 74244
• NCBI Accession #: NP_001240646.1
• NCBI GenBank Nucleotide #: NM_001253717.1
• UniProt Accession #: Q9D906; Q3TCD9; Q8K4Q5
• Molecular Weight: 77,520 Da
• NCBI Official Full Name: ubiquitin-like modifier-activating enzyme ATG7 isoform 1
• NCBI Official Synonym Full Names: autophagy related 7
• NCBI Official Symbol: Atg7
• NCBI Official Synonym Symbols: Agp7; Apg7l; Atg7l; Gm21553; 1810013K23Rik
• NCBI Protein Information: ubiquitin-like modifier-activating enzyme ATG7
• UniProt Protein Name: Ubiquitin-like modifier-activating enzyme ATG7
• Protein Family: Ubiquitin-like modifier-activating enzyme
• UniProt Gene Name: Atg7
• UniProt Synonym Gene Names: Apg7l; APG7-like; mAGP7
• UniProt Entry Name: ATG7_MOUSE

NCBI Description

This gene encodes an E1-like activating enzyme that is essential for autophagy and cytoplasmic to vacuole transport. The encoded protein is also thought to modulate p53-dependent cell cycle pathways during prolonged metabolic stress. It has been associated with multiple functions, including axon membrane trafficking, axonal homeostasis, mitophagy, adipose differentiation, and hematopoietic stem cell maintenance. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Sep 2015]

Uniprot Description

ATG7: Functions as an E1 enzyme essential for multisubstrates such as ATG8-like proteins and ATG12. Forms intermediate conjugates with ATG8-like proteins (GABARAP, GABARAPL1, GABARAPL2 or MAP1LC3A). PE-conjugation to ATG8-like proteins is essential for autophagy. Also acts as an E1 enzyme for ATG12 conjugation to ATG5 and ATG3. Homodimer. Interacts with ATG3 and ATG12. The complex, composed of ATG3 and ATG7, plays a role in the conjugation of ATG12 to ATG5. Widely expressed, especially in kidney, liver, lymph nodes and bone marrow. Belongs to the ATG7 family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Autophagy; Ubiquitin conjugating system

Cellular Component: cytoplasm; axoneme; cytosol

Molecular Function: protein binding; small protein activating enzyme activity; protein homodimerization activity; APG8 activating enzyme activity; APG12 activating enzyme activity; transcription factor binding

Biological Process: regulation of protein ubiquitination; positive regulation of apoptosis; macroautophagy; pyramidal neuron development; adult walking behavior; post-embryonic development; protein transport; cardiac muscle cell development; transport; protein amino acid lipidation; protein catabolic process; neurite development; positive regulation of autophagy; autophagic vacuole formation; amino acid metabolic process; mitochondrion organization and biogenesis; central nervous system neuron axonogenesis; organelle organization and biogenesis; cerebellar Purkinje cell layer development; C-terminal protein lipidation; cellular response to starvation; liver development; cellular response to nitrogen starvation; neurological system process; response to starvation; protein modification by small protein conjugation; mitochondrion degradation; positive regulation of protein modification process; positive regulation of protein catabolic process; autophagy; cerebral cortex development; positive regulation of macroautophagy; negative regulation of apoptosis