ATG13 sirna

ATG13 siRNA (Mouse)

Cat. Num. AAA8217751

• Gene Names: Atg13; Harbi1; D2Ertd391e; 1110053A20Rik
• Reactivity: Mouse
• Applications: RNA Interference (RNAi)
• Purity: > 97%
• Synonyms: ATG13; ATG13 siRNA (Mouse); D2ERTD391E; KIAA0652; Autophagy-related protein 13; ATG13 sirna

• Host: Synthetic
• Reactivity: Mouse
• Specificity: ATG13 siRNA (Mouse) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
• Purity/Purification: > 97%
• Form/Format: Lyophilized powder
• Sequence Length: 479

• Applicable Applications for ATG13 sirna: RNA Interference (RNAi)
• Quality Control: Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
• Directions for Use: We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
• Components: We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of mouse ATG13 gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
• Preparation and Storage: Shipped at 4 degree C. Store at -20 degree C for one year.

Related Product Information for ATG13 sirna

siRNA to inhibit ATG13 expression using RNA interference

NCBI and Uniprot Product Information

• NCBI GI #: 21704040
• NCBI GeneID: 51897
• NCBI Accession #: NP_663503.1
• NCBI GenBank Nucleotide #: NM_145528.3
• UniProt Accession #: Q91YI1; Q80TU9; A2AH18
• Molecular Weight: 52,615 Da
• NCBI Official Full Name: autophagy-related protein 13
• NCBI Official Synonym Full Names: autophagy related 13
• NCBI Official Symbol: Atg13
• NCBI Official Synonym Symbols: Harbi1; D2Ertd391e; 1110053A20Rik
• NCBI Protein Information: autophagy-related protein 13
• UniProt Protein Name: Autophagy-related protein 13
• Protein Family: Autophagy protein
• UniProt Gene Name: Atg13
• UniProt Synonym Gene Names: D2Ertd391e; Kiaa0652
• UniProt Entry Name: ATG13_MOUSE

Uniprot Description

ATG13: an autophagy factor required for autophagosome formation. Target of the TOR kinase signaling pathway that regulates autophagy through the control of the phosphorylation status of ATG13 and ULK1, and the regulation of the ATG13-ULK1-RB1CC1 complex. Under starvation conditions, is localized to puncate structures primarily representing the isolation membrane; the isolation membrane sequesters a portion of the cytoplasm resulting in autophagosome formation. Phosphorylated by ULK1 and ULK2. Phosphorylation status depends on nutrient-rich conditions; dephosphorylated during starvation or following treatment with rapamycin. Part of a complex consisting of ATG13, ULK1 and RB1CC1. Interacts with ATG101. Interacts with ULK1 and -2 via C-terminus. Three isoforms of the human protein are produced by alternative splicing.

Protein type: Vesicle; Autophagy

Cellular Component: mitochondrion; cytoplasm; cytosol

Molecular Function: protein binding; protein kinase binding

Biological Process: autophagy; autophagic vacuole formation