Rabbit anti-Human LCN2 Polyclonal Antibody | anti-LCN2 antibody

LCN2 (Neutrophil Gelatinase-associated Lipocalin, NGAL, 25kD alpha-2-microglobulin-related Subunit of MMP-9, Lipocalin-2, Oncogene 24p3, Siderocalin LCN2, p25, HNL, NGAL) (PE)

Cat. Num. AAA6384113

• Reactivity: Human
• Applications: Western Blot
• Purity: Purified by Protein A Affinity Chromatography.
• Synonyms: LCN2; Polyclonal Antibody; LCN2 (Neutrophil Gelatinase-associated Lipocalin; NGAL; 25kD alpha-2-microglobulin-related Subunit of MMP-9; Lipocalin-2; Oncogene 24p3; Siderocalin LCN2; p25; HNL; NGAL) (PE); anti-LCN2 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes human LCN2.
• Purity/Purification: Purified by Protein A Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with R-Phycoerythrin (PE).
• Sequence Length: 198

• Applicable Applications for anti-LCN2 antibody: Western Blot (WB)
• Application Notes: Applications are based on unconjugated antibody.
• Immunogen: Full length human LCN2, aa1-198 (NP_005555.1).
• Immunogen Sequence: MPLGLLWLGLALLGALHAQAQDSTSDLIPAPPLSKVPLQQNFQDNQFQGKWYVVGLAGNAILREDKDPQKMYATIYELKEDKSYNVTSVLFRKKKCDYWIRTFVPGCQPGEFTLGNIKSYPGLTSYLVRVVSTNYNQHAMVFFKKVSQNREYFKITLYGRTKELTSELKENFIRFSKYLGLPENHIVFPVPIDQCIDG
• Conjugate: PE
• Note: Preservative Free
• Preparation and Storage: Store product at 4 degree C in the dark. DO NOT FREEZE! Stable at 4 degree C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: PE conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.

Western Blot (WB)

(LCN2 rabbit polyclonal antibody. Western Blot analysis of LCN2 expression in human spleen.)

Western Blot (WB)

(Western Blot analysis of LCN2 expression in transfected 293T cell line by LCN2 polyclonal antibody. Lane 1: LCN2 transfected lysate (21.78kD). Lane 2: Non-transfected lysate.)

Related Product Information for anti-LCN2 antibody

Lipocalin 2 is from a family of proteins that are involved in the transportation of small hydrophobic molecules including steroids, retinoids, bilin and retinods. Lipocalins have been linked to many biolchemical processes such as immune response, pheromone transport, biological prostaglandin synthesis, retinoid binding, and cancer cell interactions.

Product Categories/Family for anti-LCN2 antibody

Antibodies; Transport Proteins

NCBI and Uniprot Product Information

• NCBI GI #: 5031853
• NCBI Accession #: NP_005555.1
• NCBI GenBank Nucleotide #: NM_005564.1
• UniProt Accession #: P80188
• NCBI Official Full Name: lipocalin 2 (oncogene 24p3)
• UniProt Protein Name: Neutrophil gelatinase-associated lipocalin
• Protein Family: Neutrophil gelatinase-associated lipocalin
• UniProt Gene Name: LCN2
• UniProt Synonym Gene Names: HNL; NGAL; NGAL
• UniProt Entry Name: NGAL_HUMAN

Uniprot Description

LCN2: Iron-trafficking protein involved in multiple processes such as apoptosis, innate immunity and renal development. Binds iron through association with 2,5-dihydroxybenzoic acid (2,5- DHBA), a siderophore that shares structural similarities with bacterial enterobactin, and delivers or removes iron from the cell, depending on the context. Iron-bound form (holo-24p3) is internalized following binding to the SLC22A17 (24p3R) receptor, leading to release of iron and subsequent increase of intracellular iron concentration. In contrast, association of the iron-free form (apo-24p3) with the SLC22A17 (24p3R) receptor is followed by association with an intracellular siderophore, iron chelation and iron transfer to the extracellular medium, thereby reducing intracellular iron concentration. Involved in apoptosis due to interleukin-3 (IL3) deprivation: iron-loaded form increases intracellular iron concentration without promoting apoptosis, while iron-free form decreases intracellular iron levels, inducing expression of the proapoptotic protein BCL2L11/BIM, resulting in apoptosis. Involved in innate immunity, possibly by sequestrating iron, leading to limit bacterial growth. Belongs to the calycin superfamily. Lipocalin family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Secreted; Secreted, signal peptide

Chromosomal Location of Human Ortholog: 9q34

Cellular Component: extracellular space; extracellular region; cytosol

Molecular Function: protein homodimerization activity; protease binding; transporter activity; iron ion binding

Biological Process: response to drug; cellular response to nutrient levels; cellular iron ion homeostasis; response to virus; positive regulation of cell projection organization and biogenesis; siderophore transport; innate immune response; ion transport; response to herbicide; transmembrane transport