ATF5 sirna

ATF5 siRNA (Mouse)

Cat. Num. AAA8236771

• Gene Names: Atf5; AFTA; Atf7; Atfx; ODA-10
• Reactivity: Mouse
• Applications: RNA Interference (RNAi)
• Purity: > 97%
• Synonyms: ATF5; ATF5 siRNA (Mouse); ATFX; NAP1; Cyclic AMP-dependent transcription factor ATF-5; cAMP-dependent transcription factor ATF-5; Activating transcription factor 5-alpha/beta; BZIP protein ATF7; NRIF3-associated protein; Transcription factor ATFx; Transcription factor-like protein ODA-10; ATF5 sirna

• Host: Synthetic
• Reactivity: Mouse
• Specificity: ATF5 siRNA (Mouse) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
• Purity/Purification: > 97%
• Form/Format: Lyophilized powder
• Sequence Length: 283

• Applicable Applications for ATF5 sirna: RNA Interference (RNAi)
• Quality Control: Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
• Directions for Use: We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
• Components: We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of mouse ATF5 gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
• Preparation and Storage: Shipped at 4 degree C. Store at -20 degree C for one year.

Related Product Information for ATF5 sirna

siRNA to inhibit ATF5 expression using RNA interference

NCBI and Uniprot Product Information

• NCBI GI #: 13507624
• NCBI GeneID: 107503
• NCBI Accession #: NP_109618.1
• NCBI GenBank Nucleotide #: NM_030693.2
• UniProt Accession #: O70191; Q58E51; Q99NH6
• Molecular Weight: 30,331 Da
• NCBI Official Full Name: cyclic AMP-dependent transcription factor ATF-5
• NCBI Official Synonym Full Names: activating transcription factor 5
• NCBI Official Symbol: Atf5
• NCBI Official Synonym Symbols: AFTA; Atf7; Atfx; ODA-10
• NCBI Protein Information: cyclic AMP-dependent transcription factor ATF-5
• UniProt Protein Name: Cyclic AMP-dependent transcription factor ATF-5
• Protein Family: Cyclic AMP-dependent transcription factor
• UniProt Gene Name: Atf5
• UniProt Synonym Gene Names: Atfx; Nap1; cAMP-dependent transcription factor ATF-5
• UniProt Entry Name: ATF5_MOUSE

Uniprot Description

ATF-5: Transcriptional activator which binds the cAMP response element (CRE) (consensus: 5'-GTGACGT[AC][AG]-3'), a sequence present in many viral and cellular promoters and blocks the differentiation of neuroprogenitor cells into neurons. Its transcriptional activity is enhanced by CCND3 and slightly inhibited by CDK4. Belongs to the bZIP family.

Protein type: DNA-binding

Cellular Component: nucleoplasm; transcription factor complex; cytoplasm; nucleus

Molecular Function: protein binding; DNA binding; sequence-specific DNA binding; heat shock protein binding; transcription factor activity

Biological Process: transcription, DNA-dependent; olfactory bulb interneuron development; regulation of cell cycle; multicellular organism growth; olfactory lobe development; olfactory bulb interneuron differentiation; negative regulation of neurogenesis; post-embryonic development; regulation of cell proliferation; regulation of transcription from RNA polymerase II promoter; regulation of transcription, DNA-dependent; regulation of gene expression; negative regulation of astrocyte differentiation; positive regulation of transcription from RNA polymerase II promoter; negative regulation of apoptosis