ARAP3 sirna

ARAP3 siRNA (Mouse)

Cat. Num. AAA8209486

• Gene Names: Arap3; Drag1; Centd3; cnt-d3; E030006K04Rik
• Reactivity: Mouse
• Applications: RNA Interference (RNAi)
• Purity: > 97%
• Synonyms: ARAP3; ARAP3 siRNA (Mouse); CENTD3; DRAG1; KIAA4097; Arf-GAP with Rho-GAP domain. ANK repeat and PH domain-containing protein 3; Centaurin-delta-3; Cnt-d3; Dual specificity Rho- and Arf-GTPase-activating protein 1; ARAP3 sirna

• Host: Synthetic
• Reactivity: Mouse
• Specificity: ARAP3 siRNA (Mouse) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
• Purity/Purification: > 97%
• Form/Format: Lyophilized powder
• Sequence Length: 1460

• Applicable Applications for ARAP3 sirna: RNA Interference (RNAi)
• Quality Control: Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
• Directions for Use: We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
• Components: We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of mouse ARAP3 gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
• Preparation and Storage: Shipped at 4 degree C. Store at -20 degree C for one year.

Related Product Information for ARAP3 sirna

siRNA to inhibit ARAP3 expression using RNA interference

NCBI and Uniprot Product Information

• NCBI GI #: 329663224
• NCBI GeneID: 106952
• NCBI Accession #: NP_001192265.1
• NCBI GenBank Nucleotide #: NM_001205336.1
• UniProt Accession #: Q8R5G7; Q5DTN4; Q6NVF1; Q8R5G6; E9QMK7
• Molecular Weight: 94,043 Da
• NCBI Official Full Name: arf-GAP with Rho-GAP domain, ANK repeat and PH domain-containing protein 3 isoform 2
• NCBI Official Synonym Full Names: ArfGAP with RhoGAP domain, ankyrin repeat and PH domain 3
• NCBI Official Symbol: Arap3
• NCBI Official Synonym Symbols: Drag1; Centd3; cnt-d3; E030006K04Rik
• NCBI Protein Information: arf-GAP with Rho-GAP domain, ANK repeat and PH domain-containing protein 3
• UniProt Protein Name: Arf-GAP with Rho-GAP domain, ANK repeat and PH domain-containing protein 3
• Protein Family: Arf-GAP with Rho-GAP domain, ANK repeat and PH domain-containing protein
• UniProt Gene Name: Arap3
• UniProt Synonym Gene Names: Centd3; Drag1; Kiaa4097; Cnt-d3
• UniProt Entry Name: ARAP3_MOUSE

Uniprot Description

ARAP3: Phosphatidylinositol 3,4,5-trisphosphate-dependent GTPase-activating protein that modulates actin cytoskeleton remodeling by regulating ARF and RHO family members. Is activated by phosphatidylinositol 3,4,5-trisphosphate (PtdIns(3,4,5)P3) binding. Can be activated by phosphatidylinositol 3,4-bisphosphate (PtdIns(3,4,5)P2) binding, albeit with lower efficiency. Acts on ARF6, RAC1, RHOA and CDC42. Plays a role in the internalization of anthrax toxin.

Protein type: Motility/polarity/chemotaxis; GAPs, ARF; GAPs

Cellular Component: ruffle; cell projection; cytoskeleton; membrane; lamellipodium; cytoplasm; plasma membrane; intracellular

Molecular Function: protein binding; phosphatidylinositol-3,4,5-triphosphate binding; zinc ion binding; phosphatidylinositol-3,4-bisphosphate binding; GTPase activator activity

Biological Process: negative regulation of Rac protein signal transduction; regulation of cell shape; regulation of GTPase activity; negative regulation of Rho protein signal transduction; signal transduction; negative regulation of cell migration; positive regulation of GTPase activity