Human gamma-glutamyl hydrolase (conjugase, folylpolygammaglutamyl hydrolase) ELISA Kit | GGH elisa kit

Human Gamma-glutamyl hydrolase, GGH ELISA Kit

Cat. Num. AAA911794

• Gene Names: GGH; GH
• Reactivity: Human
• Synonyms: gamma-glutamyl hydrolase (conjugase; folylpolygammaglutamyl hydrolase); Human Gamma-glutamyl hydrolase; GGH ELISA Kit; Human Gamma-glutamyl hydrolase (GGH) ELISA kit; GH; conjugase; folylpolygammaglutamyl hydrolase; gamma-glutamyl hydrolase; GGH elisa kit

• Reactivity: Human
• Specificity: This assay has high sensitivity and excellent specificity for detection of human GGH. No significant cross-reactivity or interference between human GGH and analogues was observed.
• Sequence Length: 318

• Samples: Serum, plasma, tissue homogenates, Cell lysates
• Assay Type: Sandwich
• Detection Range: 0.45 ng/ml -30 ng/ml.
• Sensitivity: The minimum detectable dose of human GGH is typically less than 0.11 ng/ml. The sensitivity of this assay, or Lower Limit of Detection (LLD) was defined as the lowest protein concentration that could be differentiated from zero. It was determined the mean O.D value of 20 replicates of the zero standard added by their three standard deviations.
• Intra-assay Precision: Intra-assay Precision (Precision within an assay): CV%<8%. Three samples of known concentration were tested twenty times on one plate to assess.
• Inter-assay Precision: Inter-assay Precision (Precision between assays): CV%<10%. Three samples of known concentration were tested in twenty assays to assess.
• Preparation and Storage: Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for GGH elisa kit

Principle of the assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for GGH has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any GGH present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for GGH is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of GGH bound in the initial step. The color development is stopped and the intensity of the color is measured.

NCBI and Uniprot Product Information

• NCBI GI #: 4503987
• NCBI GeneID: 8836
• NCBI Accession #: NP_003869.1
• NCBI GenBank Nucleotide #: NM_003878.2
• UniProt Accession #: Q92820
• Molecular Weight: 35,964 Da
• NCBI Official Full Name: gamma-glutamyl hydrolase
• NCBI Official Synonym Full Names: gamma-glutamyl hydrolase (conjugase, folylpolygammaglutamyl hydrolase)
• NCBI Official Symbol: GGH
• NCBI Official Synonym Symbols: GH
• NCBI Protein Information: gamma-glutamyl hydrolase; gamma-Glu-X carboxypeptidase
• UniProt Protein Name: Gamma-glutamyl hydrolase
• Protein Family: Gamma-glutamyl hydrolase
• UniProt Gene Name: GGH
• UniProt Entry Name: GGH_HUMAN

NCBI Description

This gene catalyzes the hydrolysis of folylpoly-gamma-glutamates and antifolylpoly-gamma-glutamates by the removal of gamma-linked polyglutamates and glutamate. [provided by RefSeq, Jul 2008]

Uniprot Description

GGH: Hydrolyzes the polyglutamate sidechains of pteroylpolyglutamates. Progressively removes gamma-glutamyl residues from pteroylpoly-gamma-glutamate to yield pteroyl-alpha- glutamate (folic acid) and free glutamate. May play an important role in the bioavailability of dietary pteroylpolyglutamates and in the metabolism of pteroylpolyglutamates and antifolates. Belongs to the peptidase C26 family.

Protein type: Cofactor and Vitamin Metabolism - folate biosynthesis; Secreted; EC 3.4.19.9; Protease; Secreted, signal peptide

Chromosomal Location of Human Ortholog: 8q12.3

Cellular Component: extracellular space; lysosome; melanosome; nucleus; cytosol

Molecular Function: omega peptidase activity; exopeptidase activity

Biological Process: response to drug; response to ethanol; response to zinc ion; glutamine metabolic process; proteolysis; response to insulin stimulus