APOC3 sirna

APOC3 siRNA (Mouse)

Cat. Num. AAA8233766

• Gene Names: Apoc3; apo-CIII; apoC-III
• Reactivity: Mouse
• Applications: RNA Interference (RNAi)
• Purity: > 97%
• Synonyms: APOC3; APOC3 siRNA (Mouse); Apolipoprotein C-III; Apo-CIII; ApoC-III; Apolipoprotein C3; APOC3 sirna

• Host: Synthetic
• Reactivity: Mouse
• Specificity: APOC3 siRNA (Mouse) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
• Purity/Purification: > 97%
• Form/Format: Lyophilized powder
• Sequence Length: 99

• Applicable Applications for APOC3 sirna: RNA Interference (RNAi)
• Quality Control: Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
• Directions for Use: We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
• Components: We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of mouse APOC3 gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
• Preparation and Storage: Shipped at 4 degree C. Store at -20 degree C for one year.

Related Product Information for APOC3 sirna

siRNA to inhibit APOC3 expression using RNA interference

NCBI and Uniprot Product Information

• NCBI GI #: 577019554
• NCBI GeneID: 11814
• NCBI Accession #: NP_001276685.1
• NCBI GenBank Nucleotide #: NM_001289756.1
• UniProt Accession #: P33622; Q8VC58; Q9CPP9
• Molecular Weight: 10,982 Da
• NCBI Official Full Name: apolipoprotein C-III isoform b
• NCBI Official Synonym Full Names: apolipoprotein C-III
• NCBI Official Symbol: Apoc3
• NCBI Official Synonym Symbols: apo-CIII; apoC-III
• NCBI Protein Information: apolipoprotein C-III
• UniProt Protein Name: Apolipoprotein C-III
• Protein Family: Apolipoprotein
• UniProt Gene Name: Apoc3
• UniProt Synonym Gene Names: Apo-CIII; ApoC-III
• UniProt Entry Name: APOC3_MOUSE

NCBI Description

This gene encodes an apolipoprotein which is the major protein component of very-low-density lipoproteins (VLDL) and a minor component of high-density lipoproteins (HDL). The encoded protein is thought to regulate the metabolism of triglyceride-rich lipoproteins and play a role in lipid storage and the mobilization of fat cells. This gene is clustered with three other apolipoprotein genes on chromosome 9 and is associated with coronary disease. Mice lacking this gene have lower levels of total cholesterol in the plasma. Mutations in the human genes causes hyperalphalipoproteinemia 2, a disorder of lipid metabolism which results in a favorable lipid profile (lower LDL-cholesterol, higher HDL-cholesterol and lower levels of serum triglycerides when fasting and after a meal). Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jan 2014]

Uniprot Description

APOC3: Inhibits lipoprotein lipase and hepatic lipase and decreases the uptake of lymph chylomicrons by hepatic cells. This suggests that it delays the catabolism of triglyceride-rich particles. Defects in APOC3 are the cause of hyperalphalipoproteinemia type 2 (HALP2). HALP2 is a condition characterized by high levels of high density lipoprotein (HDL) and increased HDL cholesterol levels. Belongs to the apolipoprotein C3 family.

Protein type: Secreted; Secreted, signal peptide; Lipid-binding

Cellular Component: extracellular space; chylomicron; cell; extracellular region

Molecular Function: lipid binding

Biological Process: response to peptide hormone stimulus; cholesterol metabolic process; triacylglycerol metabolic process; transport; lipoprotein transport; triacylglycerol catabolic process; lipoprotein metabolic process; lipid metabolic process; triacylglycerol mobilization; lipid transport; lipid catabolic process