APOBR sirna

APOBR siRNA (Mouse)

Cat. Num. AAA828340

• Gene Names: Apobr; Apob48r; Apob-48r
• Reactivity: Mouse
• Applications: RNA Interference (RNAi)
• Purity: > 97%
• Synonyms: APOBR; APOBR siRNA (Mouse); APOB48R; Apolipoprotein B receptor; Apolipoprotein B-100 receptor; Apolipoprotein B48 receptor; apoB-48R; APOBR sirna

• Host: Synthetic
• Reactivity: Mouse
• Specificity: APOBR siRNA (Mouse) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
• Purity/Purification: > 97%
• Form/Format: Lyophilized powder
• Sequence Length: 942

• Applicable Applications for APOBR sirna: RNA Interference (RNAi)
• Quality Control: Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
• Directions for Use: We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
• Components: We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of mouse APOBR gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
• Preparation and Storage: Shipped at 4 degree C. Store at -20 degree C for one year.

Related Product Information for APOBR sirna

siRNA to inhibit APOBR expression using RNA interference

NCBI and Uniprot Product Information

• NCBI GI #: 19923863
• NCBI GeneID: 171504
• NCBI Accession #: NP_612183.1
• NCBI GenBank Nucleotide #: NM_138310.1
• UniProt Accession #: Q8VBT6
• Molecular Weight: 102,705 Da
• NCBI Official Full Name: apolipoprotein B receptor
• NCBI Official Synonym Full Names: apolipoprotein B receptor
• NCBI Official Symbol: Apobr
• NCBI Official Synonym Symbols: Apob48r; Apob-48r
• NCBI Protein Information: apolipoprotein B receptor
• UniProt Protein Name: Apolipoprotein B receptor
• Protein Family: Apolipoprotein B receptor
• UniProt Gene Name: Apobr
• UniProt Synonym Gene Names: Apob48r; Apolipoprotein B48 receptor; apoB-48R
• UniProt Entry Name: APOBR_MOUSE

Uniprot Description

APOB48R: Macrophage receptor that binds to the apolipoprotein B48 (APOB) of dietary triglyceride (TG)-rich lipoproteins (TRL) or to a like domain of APOB in hypertriglyceridemic very low density lipoprotein (HTG-VLDL). Binds and internalizes TRL when out of the context of the macrophage. May provide essential lipids to reticuloendothelial cells. Could also be involved in foam cell formation with elevated TRL and remnant lipoprotein (RLP). Mediates the rapid high-affinity uptake of chylomicrons (CM), HTG- VLDL, and trypsinized (tryp) VLDL devoid of APOE in vitro in macrophages. 3 isoforms of the human protein are produced by alternative splicing.

Protein type: Receptor, misc.

Cellular Component: chylomicron; membrane; plasma membrane

Molecular Function: very-low-density lipoprotein receptor activity; lipoprotein receptor activity

Biological Process: steroid metabolic process; cholesterol metabolic process; receptor-mediated endocytosis; triacylglycerol metabolic process; transport; lipid metabolic process; lipid transport