ALOX5AP sirna

ALOX5AP siRNA (Mouse)

Cat. Num. AAA8202422

• Gene Names: Alox5ap; Flap
• Reactivity: Mouse
• Applications: RNA Interference (RNAi)
• Purity: > 97%
• Synonyms: ALOX5AP; ALOX5AP siRNA (Mouse); FLAP; Arachidonate 5-lipoxygenase-activating protein; MK-886-binding protein; ALOX5AP sirna

• Host: Synthetic
• Reactivity: Mouse
• Specificity: ALOX5AP siRNA (Mouse) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
• Purity/Purification: > 97%
• Form/Format: Lyophilized powder
• Sequence Length: 161

• Applicable Applications for ALOX5AP sirna: RNA Interference (RNAi)
• Quality Control: Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
• Directions for Use: We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
• Components: We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of mouse ALOX5AP gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
• Preparation and Storage: Shipped at 4 degree C. Store at -20 degree C for one year.

Related Product Information for ALOX5AP sirna

siRNA to inhibit ALOX5AP expression using RNA interference

NCBI and Uniprot Product Information

• NCBI GI #: 33563242
• NCBI GeneID: 11690
• NCBI Accession #: NP_033793.1
• NCBI GenBank Nucleotide #: NM_009663.2
• UniProt Accession #: P30355; Q9D138
• Molecular Weight: 18,136 Da
• NCBI Official Full Name: arachidonate 5-lipoxygenase-activating protein isoform 1
• NCBI Official Synonym Full Names: arachidonate 5-lipoxygenase activating protein
• NCBI Official Symbol: Alox5ap
• NCBI Official Synonym Symbols: Flap
• NCBI Protein Information: arachidonate 5-lipoxygenase-activating protein
• UniProt Protein Name: Arachidonate 5-lipoxygenase-activating protein
• Protein Family: Arachidonate 5-lipoxygenase-activating protein
• UniProt Gene Name: Alox5ap
• UniProt Synonym Gene Names: Flap
• UniProt Entry Name: AL5AP_MOUSE

Uniprot Description

ALOX5AP: Required for leukotriene biosynthesis by ALOX5 (5- lipoxygenase). Anchors ALOX5 to the membrane. Binds arachidonic acid, and could play an essential role in the transfer of arachidonic acid to ALOX5. Binds to MK-886, a compound that blocks the biosynthesis of leukotrienes. Genetic variations in ALOX5AP may be a cause of susceptibility to ischemic stroke (ISCHSTR); also known as cerebrovascular accident or cerebral infarction. A stroke is an acute neurologic event leading to death of neural tissue of the brain and resulting in loss of motor, sensory and/or cognitive function. Ischemic strokes, resulting from vascular occlusion, is considered to be a highly complex disease consisting of a group of heterogeneous disorders with multiple genetic and environmental risk factors. Genetic variations in ALOX5AP may be associated with susceptibility to myocardial infarction. Involvement in myocardial infarction is however unclear: according to some authors (PubMed:14770184), a 4-SNP haplotype in ALOX5AP confers risk of myocardial infarction, while according to other (PubMed:17304054) ALOX5AP is not implicated in this condition. Belongs to the MAPEG family.

Protein type: Endoplasmic reticulum; Nuclear envelope; Membrane protein, multi-pass; Membrane protein, integral; Activator

Cellular Component: nuclear membrane; membrane; endoplasmic reticulum; integral to membrane; nuclear envelope; cytosol; nucleus

Molecular Function: arachidonic acid binding; protein binding; protein homodimerization activity; enzyme binding; protein heterodimerization activity; arachidonate 5-lipoxygenase activity; enzyme activator activity; protein N-terminus binding

Biological Process: leukotriene biosynthetic process; leukotriene production during acute inflammatory response; lipoxygenase pathway; positive regulation of acute inflammatory response; leukotriene metabolic process