ALAD sirna

ALAD siRNA (Mouse)

Cat. Num. AAA8202739

• Gene Names: Alad; Lv; ALADH
• Reactivity: Mouse
• Applications: RNA Interference (RNAi)
• Purity: > 97%
• Synonyms: ALAD; ALAD siRNA (Mouse); LV; Delta-aminolevulinic acid dehydratase; ALADH; Porphobilinogen synthase; ALAD sirna

• Host: Synthetic
• Reactivity: Mouse
• Specificity: ALAD siRNA (Mouse) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
• Purity/Purification: > 97%
• Form/Format: Lyophilized powder
• Sequence Length: 330

• Applicable Applications for ALAD sirna: RNA Interference (RNAi)
• Quality Control: Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
• Directions for Use: We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
• Components: We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of mouse ALAD gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
• Preparation and Storage: Shipped at 4 degree C. Store at -20 degree C for one year.

Related Product Information for ALAD sirna

siRNA to inhibit ALAD expression using RNA interference

NCBI and Uniprot Product Information

• NCBI GI #: 451172117
• NCBI GeneID: 17025
• NCBI Accession #: NP_001263375.1
• NCBI GenBank Nucleotide #: NM_001276446.1
• UniProt Accession #: P10518; Q3THV6
• Molecular Weight: 36,024 Da
• NCBI Official Full Name: delta-aminolevulinic acid dehydratase
• NCBI Official Synonym Full Names: aminolevulinate, delta-, dehydratase
• NCBI Official Symbol: Alad
• NCBI Official Synonym Symbols: Lv; ALADH
• NCBI Protein Information: delta-aminolevulinic acid dehydratase
• UniProt Protein Name: Delta-aminolevulinic acid dehydratase
• Protein Family: Aladin
• UniProt Gene Name: Alad
• UniProt Synonym Gene Names: Lv; ALADH
• UniProt Entry Name: HEM2_MOUSE

Uniprot Description

ALAD: Catalyzes an early step in the biosynthesis of tetrapyrroles. Binds two molecules of 5-aminolevulinate per subunit, each at a distinct site, and catalyzes their condensation to form porphobilinogen. Defects in ALAD are the cause of acute hepatic porphyria (AHEPP). A form of porphyria. Porphyrias are inherited defects in the biosynthesis of heme, resulting in the accumulation and increased excretion of porphyrins or porphyrin precursors. They are classified as erythropoietic or hepatic, depending on whether the enzyme deficiency occurs in red blood cells or in the liver. AHP is characterized by attacks of gastrointestinal disturbances, abdominal colic, paralysis, and peripheral neuropathy. Most attacks are precipitated by drugs, alcohol, caloric deprivation, infections, or endocrine factors. Belongs to the ALADH family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Lyase; Cofactor and Vitamin Metabolism - porphyrin and chlorophyll; EC 4.2.1.24

Cellular Component: extracellular space; nucleus; cytosol

Molecular Function: identical protein binding; porphobilinogen synthase activity; zinc ion binding; metal ion binding; lyase activity; lead ion binding; catalytic activity

Biological Process: response to drug; response to organic substance; response to metal ion; response to ethanol; response to cadmium ion; response to zinc ion; metabolic process; response to arsenic; tetrapyrrole biosynthetic process; porphyrin biosynthetic process; response to nutrient; protein homooligomerization; heme biosynthetic process