AK1 sirna

AK1 siRNA (Mouse)

Cat. Num. AAA8219979

• Gene Names: Ak1; Ak-1; B430205N08Rik
• Reactivity: Mouse
• Applications: RNA Interference (RNAi)
• Purity: > 97%
• Synonyms: AK1; AK1 siRNA (Mouse); Adenylate kinase isoenzyme 1; AK 1; ATP-AMP transphosphorylase 1; ATP:AMP phosphotransferase; Adenylate monophosphate kinase; Myokinase; AK1 sirna

• Host: Synthetic
• Reactivity: Mouse
• Specificity: AK1 siRNA (Mouse) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
• Purity/Purification: > 97%
• Form/Format: Lyophilized powder
• Sequence Length: 194

• Applicable Applications for AK1 sirna: RNA Interference (RNAi)
• Quality Control: Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
• Directions for Use: We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
• Components: We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of mouse AK1 gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
• Preparation and Storage: Shipped at 4 degree C. Store at -20 degree C for one year.

Related Product Information for AK1 sirna

siRNA to inhibit AK1 expression using RNA interference

NCBI and Uniprot Product Information

• NCBI GI #: 311771688
• NCBI GeneID: 11636
• NCBI Accession #: NP_001185719.1
• NCBI GenBank Nucleotide #: NM_001198790.1
• UniProt Accession #: Q9R0Y5; Q542C5; Q9R0Y4; A2AK80
• Molecular Weight: 23,116 Da
• NCBI Official Full Name: adenylate kinase isoenzyme 1 isoform 2
• NCBI Official Synonym Full Names: adenylate kinase 1
• NCBI Official Symbol: Ak1
• NCBI Official Synonym Symbols: Ak-1; B430205N08Rik
• NCBI Protein Information: adenylate kinase isoenzyme 1
• UniProt Protein Name: Adenylate kinase isoenzyme 1
• Protein Family: Adenylate kinase
• UniProt Gene Name: Ak1
• UniProt Synonym Gene Names: AK 1
• UniProt Entry Name: KAD1_MOUSE

Uniprot Description

AK1: Catalyzes the reversible transfer of the terminal phosphate group between ATP and AMP. Small ubiquitous enzyme involved in energy metabolism and nucleotide synthesis that is essential for maintenance and cell growth. Defects in AK1 are the cause of hemolytic anemia due to adenylate kinase deficiency (HAAKD). Belongs to the adenylate kinase family.

Protein type: EC 2.7.4.6; Nucleotide Metabolism - purine; Kinase, other; EC 2.7.4.3

Cellular Component: sarcomere; neuron projection; perinuclear region of cytoplasm; cytoplasm; plasma membrane; outer dense fiber

Molecular Function: transferase activity; nucleobase, nucleoside, nucleotide kinase activity; nucleotide binding; nucleoside diphosphate kinase activity; adenylate kinase activity; kinase activity; ATP binding; phosphotransferase activity, phosphate group as acceptor

Biological Process: ATP metabolic process; nucleobase, nucleoside, nucleotide and nucleic acid metabolic process; nucleoside triphosphate biosynthetic process; nucleoside diphosphate phosphorylation; ADP biosynthetic process; inosine biosynthetic process; AMP metabolic process; cell cycle arrest; phosphorylation; nucleotide phosphorylation