ABL1 sirna

ABL1 siRNA (Mouse)

Cat. Num. AAA8230787

• Gene Names: Abl1; Abl; c-Abl; AI325092; E430008G22Rik
• Reactivity: Mouse
• Applications: RNA Interference (RNAi)
• Purity: > 97%
• Synonyms: ABL1; ABL1 siRNA (Mouse); ABL; Tyrosine-protein kinase ABL1; Abelson murine leukemia viral oncogene homolog 1; Abelson tyrosine-protein kinase 1; Proto-oncogene c-Abl; p150; ABL1 sirna

• Host: Synthetic
• Reactivity: Mouse
• Specificity: ABL1 siRNA (Mouse) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
• Purity/Purification: > 97%
• Form/Format: Lyophilized powder
• Sequence Length: 1142

• Applicable Applications for ABL1 sirna: RNA Interference (RNAi)
• Quality Control: Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
• Directions for Use: We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
• Components: We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of mouse ABL1 gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
• Preparation and Storage: Shipped at 4 degree C. Store at -20 degree C for one year.

Related Product Information for ABL1 sirna

siRNA to inhibit ABL1 expression using RNA interference

NCBI and Uniprot Product Information

• NCBI GI #: 162951870
• NCBI GeneID: 11350
• NCBI Accession #: NP_001106174.1
• NCBI GenBank Nucleotide #: NM_001112703.2
• UniProt Accession #: P00520; P97896; Q61252; Q61253; Q61254; Q61255; Q61256; Q61257; Q61258; Q61259; Q61260; Q61261
• Molecular Weight: 124,769 Da
• NCBI Official Full Name: tyrosine-protein kinase ABL1 isoform a
• NCBI Official Synonym Full Names: c-abl oncogene 1, non-receptor tyrosine kinase
• NCBI Official Symbol: Abl1
• NCBI Official Synonym Symbols: Abl; c-Abl; AI325092; E430008G22Rik
• NCBI Protein Information: tyrosine-protein kinase ABL1
• UniProt Protein Name: Tyrosine-protein kinase ABL1
• Protein Family: Tyrosine-protein kinase
• UniProt Gene Name: Abl1
• UniProt Synonym Gene Names: Abl
• UniProt Entry Name: ABL1_MOUSE

Uniprot Description

Abl: an ubiquitously expressed and highly conserved proto-oncogenic tyrosine kinase. c-Abl protein is distributed in both the nucleus and the cytoplasm of cells. Negatively regulated by its SH3 domain, and deletion of the SH3 domain turns ABL1 into an oncogene. It has been implicated in regulation of cell proliferation, differentiation, apoptosis, cell adhesion and stress response. The Philadephia chromosome translocation t(9;22)(q34;q11) creates a Bcr-Abl fusion protein, responsible for 90% of chronic myelogenous leukemia (CML) and ~25% of acute lymphoblastic leukemia (ALL). Inhibitors: Gleevec (imatinib, Glivec), Dasatinib. Two alternatively-spliced isoforms have been described.

Protein type: Protein kinase, tyrosine (non-receptor); EC 2.7.10.2; Oncoprotein; Protein kinase, TK; Kinase, protein; TK group; Abl family

Cellular Component: neuron projection; mitochondrion; endoplasmic reticulum; leading edge; cytosol; actin cytoskeleton; nucleoplasm; ruffle; extrinsic to internal side of plasma membrane; cytoskeleton; growth cone; cell soma; perinuclear region of cytoplasm; cytoplasm; nucleolus; synapse; nucleus

Molecular Function: protein C-terminus binding; protein domain specific binding; metal ion binding; nucleotide binding; magnesium ion binding; non-membrane spanning protein tyrosine kinase activity; protein kinase binding; protein kinase activity; transferase activity; actin filament binding; protein binding; syntaxin binding; DNA binding; manganese ion binding; protein-tyrosine kinase activity; transferase activity, transferring phosphorus-containing groups; mitogen-activated protein kinase binding; kinase activity; SH3 domain binding; ATP binding; receptor binding

Biological Process: B cell proliferation; peptidyl-tyrosine phosphorylation; DNA damage induced protein phosphorylation; apoptosis; regulation of cell cycle; positive regulation of apoptosis; alpha-beta T cell differentiation; cerebellum morphogenesis; platelet-derived growth factor receptor signaling pathway; positive regulation of mitotic cell cycle; protein amino acid phosphorylation; negative regulation of BMP signaling pathway; transitional one stage B cell differentiation; elevation of cytosolic calcium ion concentration; B cell receptor signaling pathway; Bergmann glial cell differentiation; collateral sprouting; negative regulation of mitotic cell cycle; neuromuscular process controlling balance; cell adhesion; B cell proliferation during immune response; positive regulation of oxidoreductase activity; epidermal growth factor receptor signaling pathway; spleen development; cell migration; positive regulation of I-kappaB kinase/NF-kappaB cascade; thymus development; B-1 B cell homeostasis; activated T cell proliferation; DNA damage response, signal transduction; endocytosis; negative regulation of I-kappaB kinase/NF-kappaB cascade; DNA repair; phagocytosis; positive regulation of peptidyl-tyrosine phosphorylation; regulation of actin cytoskeleton organization and biogenesis; microspike biogenesis; positive regulation of osteoblast proliferation; negative regulation of ubiquitin-protein ligase activity; autophagy; innate immune response; positive regulation of release of sequestered calcium ion into cytosol; negative regulation of cell-cell adhesion; response to oxidative stress; actin cytoskeleton organization and biogenesis; response to DNA damage stimulus; phosphorylation