ABCC6 sirna

ABCC6 siRNA (Mouse)

Cat. Num. AAA8210394

• Gene Names: Abcc6; DCC; Mrp6; Abcc1b; dyscalc; Dyscalc1
• Reactivity: Mouse
• Applications: RNA Interference (RNAi)
• Purity: > 97%
• Synonyms: ABCC6; ABCC6 siRNA (Mouse); MRP6; Multidrug resistance-associated protein 6; ATP-binding cassette sub-family C member 6; ABCC6 sirna

• Host: Synthetic
• Reactivity: Mouse
• Specificity: ABCC6 siRNA (Mouse) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
• Purity/Purification: > 97%
• Form/Format: Lyophilized powder
• Sequence Length: 1498

• Applicable Applications for ABCC6 sirna: RNA Interference (RNAi)
• Quality Control: Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
• Directions for Use: We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
• Components: We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of mouse ABCC6 gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
• Preparation and Storage: Shipped at 4 degree C. Store at -20 degree C for one year.

Related Product Information for ABCC6 sirna

siRNA to inhibit ABCC6 expression using RNA interference

NCBI and Uniprot Product Information

• NCBI GI #: 145275191
• NCBI GeneID: 27421
• NCBI Accession #: NP_061265.2
• NCBI GenBank Nucleotide #: NM_018795.2
• UniProt Accession #: Q9R1S7; Q80YB6; A2TAJ0; A2TAJ1; F8VPT2
• Molecular Weight: 164,857 Da
• NCBI Official Full Name: multidrug resistance-associated protein 6
• NCBI Official Synonym Full Names: ATP-binding cassette, sub-family C (CFTR/MRP), member 6
• NCBI Official Symbol: Abcc6
• NCBI Official Synonym Symbols: DCC; Mrp6; Abcc1b; dyscalc; Dyscalc1
• NCBI Protein Information: multidrug resistance-associated protein 6
• UniProt Protein Name: Multidrug resistance-associated protein 6
• Protein Family: ABC transporter C family
• UniProt Gene Name: Abcc6
• UniProt Synonym Gene Names: Mrp6
• UniProt Entry Name: MRP6_MOUSE

NCBI Description

The protein encoded by this gene is a member of the superfamily of ATP-binding cassette (ABC) transporters. ABC proteins transport various molecules across extra- and intra-cellular membranes. ABC genes are divided into seven distinct subfamilies (ABC1, MDR/TAP, MRP, ALD, OABP, GCN20, White). This protein is a member of the MRP subfamily which is involved in multi-drug resistance. The specific function of this protein is unknown; however, a similar rat protein has been identified as the major canalicular bile salt export pump of liver. [provided by RefSeq, Jul 2008]

Uniprot Description

ABCC6: May participate directly in the active transport of drugs into subcellular organelles or influence drug distribution indirectly. Transports glutathione conjugates as leukotriene-c4 (LTC4) and N-ethylmaleimide S-glutathione (NEM-GS). Defects in ABCC6 are the cause of pseudoxanthoma elasticum (PXE). PXE is a disorder characterized by calcification of elastic fibers in skin, arteries and retina that results in dermal lesions with associated laxity and loss of elasticity, arterial insufficiency and retinal hemorrhages leading to macular degeneration. PXE is caused in the overwhelming majority of cases by homozygous or compound heterozygous mutations in the ABCC6 gene (autosomal recessive PXE). Individuals carrying heterozygous mutations express limited manifestations of the pseudoxanthoma elasticum phenotype (autosomal dominant PXE). Defects in ABCC6 are the cause of arterial calcification of infancy, generalized, type 2 (GACI2). GACI2 is a severe autosomal recessive disorder characterized by calcification of the internal elastic lamina of muscular arteries and stenosis due to myointimal proliferation. The disorder is often fatal within the first 6 months of life because of myocardial ischemia resulting in refractory heart failure. Belongs to the ABC transporter superfamily. ABCC family. Conjugate transporter (TC 3.A.1.208) subfamily.

Protein type: Transporter, ABC family; Membrane protein, integral; Membrane protein, multi-pass; Hydrolase; Transporter

Cellular Component: membrane; apical plasma membrane; plasma membrane; integral to membrane; nucleus; lateral plasma membrane

Molecular Function: ATPase activity, coupled to transmembrane movement of substances; ATPase activity; nucleotide binding; ATP binding

Biological Process: transport; metabolic process; transmembrane transport