ABCB9 sirna

ABCB9 siRNA (Mouse)

Cat. Num. AAA8202900

• Gene Names: Abcb9; TAPL; mKIAA1520
• Reactivity: Mouse
• Applications: RNA Interference (RNAi)
• Purity: > 97%
• Synonyms: ABCB9; ABCB9 siRNA (Mouse); KIAA1520; ATP-binding cassette sub-family B member 9; ATP-binding cassette transporter 9; ABC transporter 9 protein; mABCB9; TAP-like protein; TAPL; ABCB9 sirna

• Host: Synthetic
• Reactivity: Mouse
• Specificity: ABCB9 siRNA (Mouse) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
• Purity/Purification: > 97%
• Form/Format: Lyophilized powder
• Sequence Length: 762

• Applicable Applications for ABCB9 sirna: RNA Interference (RNAi)
• Quality Control: Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
• Directions for Use: We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
• Components: We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of mouse ABCB9 gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
• Preparation and Storage: Shipped at 4 degree C. Store at -20 degree C for one year.

Related Product Information for ABCB9 sirna

siRNA to inhibit ABCB9 expression using RNA interference

NCBI and Uniprot Product Information

• NCBI GI #: 66932952
• NCBI GeneID: 56325
• NCBI Accession #: NP_063928.2
• NCBI GenBank Nucleotide #: NM_019875.2
• UniProt Accession #: Q9JJ59; Q8CHA1; Q9D212; Q9JIN1
• Molecular Weight: 70,216 Da
• NCBI Official Full Name: ATP-binding cassette sub-family B member 9
• NCBI Official Synonym Full Names: ATP-binding cassette, sub-family B (MDR/TAP), member 9
• NCBI Official Symbol: Abcb9
• NCBI Official Synonym Symbols: TAPL; mKIAA1520
• NCBI Protein Information: ATP-binding cassette sub-family B member 9
• UniProt Protein Name: ATP-binding cassette sub-family B member 9
• Protein Family: ABC transporter B family
• UniProt Gene Name: Abcb9
• UniProt Synonym Gene Names: Kiaa1520; ABC transporter 9 protein; mABCB9; TAPL
• UniProt Entry Name: ABCB9_MOUSE

NCBI Description

The membrane-associated protein encoded by this gene is a member of the superfamily of ATP-binding cassette (ABC) transporters. ABC proteins transport various molecules across extra- and intra-cellular membranes. ABC genes are divided into seven distinct subfamilies (ABC1, MDR/TAP, MRP, ALD, OABP, GCN20, White). This protein is a member of the MDR/TAP subfamily. Members of the MDR/TAP subfamily are involved in multidrug resistance as well as antigen presentation. The function of this half-transporter has not yet been determined; however, it may be associated with lysosome activity. [provided by RefSeq, Jul 2008]

Uniprot Description

ABCB9: ATP-dependent low-affinity peptide transporter which translocates a broad spectrum of peptides from the cytosol to the lysosomal lumen. Displays a broad peptide length specificity from 6-mer up to at least 59-mer peptides with an optimum of 23-mers. Favors positively charged, aromatic or hydrophobic residues in the N- and C-terminal positions whereas negatively charged residues as well as asparagine and methionine are not favored. Belongs to the ABC transporter superfamily. ABCB family. MHC peptide exporter (TC 3.A.1.209) subfamily. 5 isoforms of the human protein are produced by alternative splicing.

Protein type: Membrane protein, integral; Transporter; Membrane protein, multi-pass; Transporter, ABC family

Cellular Component: endoplasmic reticulum membrane; membrane; lysosome; lysosomal membrane; integral to membrane; integral to endoplasmic reticulum membrane

Molecular Function: protein homodimerization activity; ATPase activity, coupled to transmembrane movement of substances; ATPase activity; nucleotide binding; substrate-specific transmembrane transporter activity; ATP binding; peptide-transporting ATPase activity

Biological Process: peptide transport; protein transport; transport; transmembrane transport