AARS2 sirna

AARS2 siRNA (Mouse)

Cat. Num. AAA8204701

• Gene Names: Aars2; Gm89; Aarsl; AlaRS
• Reactivity: Mouse
• Applications: RNA Interference (RNAi)
• Purity: > 97%
• Synonyms: AARS2; AARS2 siRNA (Mouse); AARSL; GM89; KIAA1270; Alanine--tRNA ligase mitochondrial; Alanyl-tRNA synthetase; AlaRS; AARS2 sirna

• Host: Synthetic
• Reactivity: Mouse
• Specificity: AARS2 siRNA (Mouse) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
• Purity/Purification: > 97%
• Form/Format: Lyophilized powder
• Sequence Length: 980

• Applicable Applications for AARS2 sirna: RNA Interference (RNAi)
• Quality Control: Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
• Directions for Use: We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
• Components: We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of mouse AARS2 gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
• Preparation and Storage: Shipped at 4 degree C. Store at -20 degree C for one year.

Related Product Information for AARS2 sirna

siRNA to inhibit AARS2 expression using RNA interference

NCBI and Uniprot Product Information

• NCBI GI #: 52630311
• NCBI GeneID: 224805
• NCBI Accession #: NP_941010.2
• NCBI GenBank Nucleotide #: NM_198608.2
• UniProt Accession #: Q14CH7; Q68FH3; Q69ZM9
• Molecular Weight: 106,783 Da
• NCBI Official Full Name: alanine--tRNA ligase, mitochondrial
• NCBI Official Synonym Full Names: alanyl-tRNA synthetase 2, mitochondrial (putative)
• NCBI Official Symbol: Aars2
• NCBI Official Synonym Symbols: Gm89; Aarsl; AlaRS
• NCBI Protein Information: alanine--tRNA ligase, mitochondrial
• UniProt Protein Name: Alanine--tRNA ligase, mitochondrial
• UniProt Gene Name: Aars2
• UniProt Synonym Gene Names: Aarsl; Gm89; Kiaa1270; AlaRS
• UniProt Entry Name: SYAM_MOUSE

Uniprot Description

AARSL: Catalyzes the attachment of alanine to tRNA(Ala) in a two-step reaction: alanine is first activated by ATP to form Ala- AMP and then transferred to the acceptor end of tRNA(Ala). Also edits incorrectly charged tRNA(Ala) via its editing domain. Defects in AARS2 are the cause of combined oxidative phosphorylation deficiency type 8 (COXPD8). A mitochondrial disease characterized by a lethal infantile hypertrophic cardiomyopathy, generalized muscle dysfunction and some neurologic involvement. The liver is not affected. Belongs to the class-II aminoacyl-tRNA synthetase family.

Protein type: EC 6.1.1.7; Translation; Ligase

Cellular Component: mitochondrion; cytoplasm

Molecular Function: aminoacyl-tRNA ligase activity; nucleic acid binding; RNA binding; metal ion binding; ligase activity, forming aminoacyl-tRNA and related compounds; nucleotide binding; ATP binding; alanine-tRNA ligase activity; ligase activity; tRNA binding

Biological Process: mitochondrial respiratory chain complex assembly; translation; tRNA aminoacylation; alanyl-tRNA aminoacylation