Western Blot (WB)
(Western Blot Positive WB detected in: Hela whole cell lysate, Jurkat whole cell lysate, MCF-7 whole cell lysate, RAW264.7 whole cell lysate, HepG2 whole cell lysate, PC-3 whole cell lysate, Raji whole cell lysate, 293 whole cell lysate, Mouse liver tissue All lanes: TPT1 antibody at 1:2000 Secondary Goat polyclonal to rabbit IgG at 1/50000 dilution Predicted band size: 20, 16 kDa Observed band size: 25 kDa)
TPT1 recombinant antibody
TPT1 Antibody
IHC: 1:50-1:200
IF: 1:20-1:200
IP: 1:200-1:1000
Western Blot (WB)
(Western Blot Positive WB detected in: Hela whole cell lysate, Jurkat whole cell lysate, MCF-7 whole cell lysate, RAW264.7 whole cell lysate, HepG2 whole cell lysate, PC-3 whole cell lysate, Raji whole cell lysate, 293 whole cell lysate, Mouse liver tissue All lanes: TPT1 antibody at 1:2000 Secondary Goat polyclonal to rabbit IgG at 1/50000 dilution Predicted band size: 20, 16 kDa Observed band size: 25 kDa)
Western Blot (WB)
(Western Blot Positive WB detected in: Hela whole cell lysate, Jurkat whole cell lysate, MCF-7 whole cell lysate, RAW264.7 whole cell lysate, HepG2 whole cell lysate, PC-3 whole cell lysate, Raji whole cell lysate, 293 whole cell lysate, Mouse liver tissue All lanes: TPT1 antibody at 1:2000 Secondary Goat polyclonal to rabbit IgG at 1/50000 dilution Predicted band size: 20, 16 kDa Observed band size: 25 kDa)
Immunohistochemistry (IHC)
(IHC image diluted at 1:100 and staining in paraffin-embedded human prostate tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4 degree C overnight. The primary is detected by a Goat anti-rabbit IgG polymer labeled by HRP and visualized using 0.05% DAB.)
Immunohistochemistry (IHC)
(IHC image diluted at 1:100 and staining in paraffin-embedded human prostate tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4 degree C overnight. The primary is detected by a Goat anti-rabbit IgG polymer labeled by HRP and visualized using 0.05% DAB.)
Immunofluorescence (IF)
(Immunofluorescence staining of SY5Y Cells at 1?50, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeated by 0.2% TritonX-100, and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4 degree C. Nuclear DNA was labeled in blue with DAPI. The secondary antibody was FITC-conjugated AffiniPure Goat Anti-Rabbit IgG ?H+L?.)
Immunofluorescence (IF)
(Immunofluorescence staining of SY5Y Cells at 1?50, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeated by 0.2% TritonX-100, and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4 degree C. Nuclear DNA was labeled in blue with DAPI. The secondary antibody was FITC-conjugated AffiniPure Goat Anti-Rabbit IgG ?H+L?.)
Immunorecipitation (IP)
(Immunoprecipitating TCTP in Hela whole cell lysate Lane 1: Rabbit control IgG instead of in Hela whole cell lysate. For western blotting,a HRP-conjugated Protein G antibody was used as the secondary antibody (1/2000) Lane 2: Hela whole cell lysate?500ug? Lane 3: Hela whole cell lysate (10ug))
Immunorecipitation (IP)
(Immunoprecipitating TCTP in Hela whole cell lysate Lane 1: Rabbit control IgG instead of in Hela whole cell lysate. For western blotting,a HRP-conjugated Protein G antibody was used as the secondary antibody (1/2000) Lane 2: Hela whole cell lysate?500ug? Lane 3: Hela whole cell lysate (10ug))
NCBI and Uniprot Product Information
Uniprot Description
TPT1: a novel heat shock protein with antiapoptotic and chaperone-like activity. Its levels are highly regulated in response to various cellular stimuli and stresses. Downregulated in response to proapoptotic treatments that induce Ca(++) stress, in a PKR-dependent manner. Colocalizes with CHFR to the mitotic spindle. Appears to be involved in calcium binding and microtubule stabilization. Its exosomal secretion is induced following DNA damage. Interacts with MCL1 and STEAP3, a p53-inducible transmembrane protein that facilitates the exosomal secretion of TPT1. Secreted TPT1 acts as a histamine-releasing factor, participates in inflammatory responses by inducing the release of histamine, and may play a role in tumor reversion. May play a direct role in placental calcium transport. Phosphorylation by Plk may decrease its microtubule-stabilizing activity and promote microtubule dynamics following metaphase. Critical for peripheral T cell maintenance and TCR-mediated cell proliferation.
Protein type: Microtubule-binding; Calcium-binding
Chromosomal Location of Human Ortholog: 13q14
Cellular Component: multivesicular body; extracellular space; tubulin complex; cytoplasm; nucleus
Molecular Function: protein binding; calcium ion binding
Biological Process: cellular calcium ion homeostasis; regulation of apoptosis; response to virus; calcium ion transport; negative regulation of apoptosis
Research Articles on TPT1
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Product Notes
The TPT1 tpt1 (Catalog #AAA7136546) is a Recombinant Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The TPT1 Antibody reacts with Human, Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's TPT1 can be used in a range of immunoassay formats including, but not limited to, ELISA (EIA), Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Immunoprecipitation (IP). WB: 1:500-1:5000 IHC: 1:50-1:200 IF: 1:20-1:200 IP: 1:200-1:1000. Researchers should empirically determine the suitability of the TPT1 tpt1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "TPT1, Monoclonal Recombinant Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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