ZNRF3 sirna

ZNRF3 siRNA (Human)

Cat. Num. AAA8229758

• Gene Names: ZNRF3; RNF203; BK747E2.3
• Reactivity: Human
• Applications: RNA Interference (RNAi)
• Purity: > 97%
• Synonyms: ZNRF3; ZNRF3 siRNA (Human); KIAA1133; RNF203; E3 ubiquitin-protein ligase ZNRF3; RING finger protein 203; Zinc/RING finger protein 3; ZNRF3 sirna

• Host: Synthetic
• Reactivity: Human
• Specificity: ZNRF3 siRNA (Human) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
• Purity/Purification: > 97%
• Form/Format: Lyophilized powder
• Sequence Length: 936

• Applicable Applications for ZNRF3 sirna: RNA Interference (RNAi)
• Quality Control: Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
• Directions for Use: We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
• Components: We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of human ZNRF3 gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
• Preparation and Storage: Shipped at 4 degree C. Store at -20 degree C for one year.

Related Product Information for ZNRF3 sirna

siRNA to inhibit ZNRF3 expression using RNA interference

NCBI and Uniprot Product Information

• NCBI GI #: 332801080
• NCBI GeneID: 84133
• NCBI Accession #: NP_001193927.1
• NCBI GenBank Nucleotide #: NM_001206998.1
• UniProt Accession #: Q9ULT6; Q6ICH1; Q6NTF8; Q8WU18; B3KU18
• Molecular Weight: 89,908 Da
• NCBI Official Full Name: E3 ubiquitin-protein ligase ZNRF3 isoform 1
• NCBI Official Synonym Full Names: zinc and ring finger 3
• NCBI Official Symbol: ZNRF3
• NCBI Official Synonym Symbols: RNF203; BK747E2.3
• NCBI Protein Information: E3 ubiquitin-protein ligase ZNRF3
• UniProt Protein Name: E3 ubiquitin-protein ligase ZNRF3
• Protein Family: E3 ubiquitin-protein ligase
• UniProt Gene Name: ZNRF3
• UniProt Synonym Gene Names: KIAA1133; RNF203
• UniProt Entry Name: ZNRF3_HUMAN

Uniprot Description

ZNRF3: E3 ubiquitin-protein ligase that acts as a negative regulator of the Wnt signaling pathway by mediating the ubiquitination and subsequent degradation of Wnt receptor complex components Frizzled and LRP6. Acts on both canonical and non- canonical Wnt signaling pathway. Acts as a tumor suppressor in the intestinal stem cell zone by inhibiting the Wnt signaling pathway, thereby resticting the size of the intestinal stem cell zone. Belongs to the ZNRF3 family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: EC 6.3.2.-; Ubiquitin conjugating system; EC 6.3.2.19; Ubiquitin ligase; Membrane protein, integral

Chromosomal Location of Human Ortholog: 22q12.1

Cellular Component: integral to plasma membrane; plasma membrane

Molecular Function: protein binding; zinc ion binding; frizzled binding; ubiquitin-protein ligase activity; ligase activity

Biological Process: ubiquitin-dependent protein catabolic process; Wnt receptor signaling pathway, planar cell polarity pathway; protein ubiquitination; Wnt receptor signaling pathway through beta-catenin