ZFYVE9 sirna

ZFYVE9 siRNA (Human)

Cat. Num. AAA8203571

• Gene Names: ZFYVE9; NSP; SARA; MADHIP; SMADIP; PPP1R173
• Reactivity: Human
• Applications: RNA Interference (RNAi)
• Purity: > 97%
• Synonyms: ZFYVE9; ZFYVE9 siRNA (Human); MADHIP; SARA; SMADIP; Zinc finger FYVE domain-containing protein 9; Mothers against decapentaplegic homolog-interacting protein; Madh-interacting protein; Novel serine protease; NSP; Receptor activation anchor; hSARA; Smad anchor for receptor activation; ZFYVE9 sirna

• Host: Synthetic
• Reactivity: Human
• Specificity: ZFYVE9 siRNA (Human) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
• Purity/Purification: > 97%
• Form/Format: Lyophilized powder
• Sequence Length: 1425

• Applicable Applications for ZFYVE9 sirna: RNA Interference (RNAi)
• Quality Control: Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
• Directions for Use: We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
• Components: We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of human ZFYVE9 gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
• Preparation and Storage: Shipped at 4 degree C. Store at -20 degree C for one year.

Related Product Information for ZFYVE9 sirna

siRNA to inhibit ZFYVE9 expression using RNA interference

NCBI and Uniprot Product Information

• NCBI GI #: 47717106
• NCBI GeneID: 9372
• NCBI Accession #: NP_004790.2
• NCBI GenBank Nucleotide #: NM_004799.3
• UniProt Accession #: O95405; Q5T0F6; Q5T0F7; Q9UNE1; Q9Y5R7
• Molecular Weight: 83,721 Da
• NCBI Official Full Name: zinc finger FYVE domain-containing protein 9 isoform 3
• NCBI Official Synonym Full Names: zinc finger, FYVE domain containing 9
• NCBI Official Symbol: ZFYVE9
• NCBI Official Synonym Symbols: NSP; SARA; MADHIP; SMADIP; PPP1R173
• NCBI Protein Information: zinc finger FYVE domain-containing protein 9
• UniProt Protein Name: Zinc finger FYVE domain-containing protein 9
• Protein Family: Zinc finger FYVE domain-containing protein
• UniProt Gene Name: ZFYVE9
• UniProt Synonym Gene Names: MADHIP; SARA; SMADIP; Madh-interacting protein; NSP; hSARA
• UniProt Entry Name: ZFYV9_HUMAN

NCBI Description

This gene encodes a double zinc finger motif-containing protein that participates in the transforming growth factor-beta (TGFB) signalling pathway. The encoded protein interacts directly with SMAD2 and SMAD3, and recruits SMAD2 to the TGFB receptor. There are multiple pseudogenes for this gene on chromosomes 2, 15, and X. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Mar 2013]

Uniprot Description

SARA: Early endosomal protein that functions to recruit SMAD2/SMAD3 to intracellular membranes and to the TGF-beta receptor. Plays a significant role in TGF-mediated signaling by regulating the subcellular location of SMAD2 and SMAD3 and modulating the transcriptional activity of the SMAD3/SMAD4 complex. Possibly associated with TGF-beta receptor internalization. 3 isoforms of the human protein are produced by alternative splicing.

Protein type: Adaptor/scaffold

Chromosomal Location of Human Ortholog: 1p32.3

Cellular Component: early endosome membrane; early endosome

Molecular Function: protein binding; serine-type peptidase activity; metal ion binding; phosphatidylinositol binding

Biological Process: transforming growth factor beta receptor signaling pathway; endocytosis; SMAD protein nuclear translocation; proteolysis; negative regulation of transforming growth factor beta receptor signaling pathway; SMAD protein complex assembly